Anti-FOXL2 (phospho S263) antibody (ab74144)


  • Product nameAnti-FOXL2 (phospho S263) antibody
    See all FOXL2 primary antibodies
  • Description
    Rabbit polyclonal to FOXL2 (phospho S263)
  • SpecificityThe antibody detects endogenous levels of FOXL2 when phosphorylated at serine 263.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized phosphopeptide derived from human FOXL2 around the phosphorylation site of serine 263 (V-Q-SP-M-A).

  • Positive control
    • K562 cells treated with Na3VO4 (0.3nM, 40mins)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab74144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
ELISA 1/20000.


  • FunctionTranscriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
  • Tissue specificityIn addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.
  • Involvement in diseaseDefects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
    Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3) [MIM:608996]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Post-translational
    Sumoylated by SUMO1; sumoylation is required for transcriptional repression activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blepharophimosis antibody
    • Blepharophimosis epicanthus inversus and ptosis 1 antibody
    • Blepharophimosis epicanthus inversus and ptosis antibody
    • BPES 1 antibody
    • BPES antibody
    • BPES1 antibody
    • Epicanthus inversus and ptosis 1 antibody
    • Forkhead box L2 antibody
    • Forkhead box protein L2 antibody
    • Forkhead transcription factor FOXL2 antibody
    • FOX L2 antibody
    • FOXL 2 antibody
    • FOXL2 antibody
    • FOXL2_HUMAN antibody
    • PFRK antibody
    • PINTO antibody
    • POF 3 antibody
    • POF3 antibody
    see all

Anti-FOXL2 (phospho S263) antibody images

  • All lanes : Anti-FOXL2 (phospho S263) antibody (ab74144) at 1/500 dilution

    Lane 1 : K562 cells treated with Na3VO4 (0.3nM, 40mins),
    Lane 2 : K562 cells treated with Na3VO4 (0.3nM, 40mins), with phosphopeptide at 10 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa

References for Anti-FOXL2 (phospho S263) antibody (ab74144)

ab74144 has not yet been referenced specifically in any publications.

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Thank you very much for your interest in ab74144. To our knowledge, ab74144 has not been tested in chicken. We do not have any trial sized or small aliquots. Therefore, I can offer a discount off a future purchase if you buy ab74144 now, test it in chi...

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