The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 - 30 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Transcriptional regulator involved in development.
Expressed in thymus.
Involvement in disease
Defects in FOXN1 are the cause of T-cell immunodeficiency congenital alopecia and nail dystrophy (TIDAND) [MIM:601705]. A disorder characterized by the association of congenital alopecia, severe T-cell immunodeficiency, and ridging and pitting of all nails.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXN1 antibody (ab26069)Image is courtesy of an AbReview submitted by Muriel Cario-Andre.
Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human skin tissue sections, labelling FOXN1 with ab26069 at a dilution of 1/25 incubated for 18 hours at 4°C. Permeablization was with 0.05% Tween. Blocking was with 5% serum incubated for 20 minutes at room temperature. The secondary used was a swine polyclonal biotin-conjugate at 1/1000.