Anti-FOXN4 antibody (ab98860)

Overview

  • Product nameAnti-FOXN4 antibody
    See all FOXN4 primary antibodies
  • Description
    Rabbit polyclonal to FOXN4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 431-480 of Human FOXN4 (NP_998761).

  • Positive control
    • OVCAR-3 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab98860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTranscription factor essential for neural and some non-neural tissues development, such as retina and lung respectively. Binds to an 11-bp consensus sequence containing the invariant tetranucleotide 5'-ACGC-3'. During development of the central nervous system, is required to specify the amacrine and horizontal cell fates from multipotent retinal progenitors while suppressing the alternative photoreceptor cell fates through activating DLL4-NOTCH signaling. Also acts synergistically with ASCL1/MASH1 to activate DLL4-NOTCH signaling and drive commitment of p2 progenitors to the V2b interneuron fates during spinal cord neurogenesis. In development of non-neural tissues, plays an essential role in the specification of the atrioventricular canal and is indirectly required for patterning the distal airway during lung development.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ35967 antibody
    • Forkhead box N4 antibody
    • Forkhead box protein N4 antibody
    • Forkhead/winged helix transcription factor FOXN4 antibody
    • FOXN4 antibody
    • FOXN4_HUMAN antibody
    see all

Anti-FOXN4 antibody images

  • Anti-FOXN4 antibody (ab98860) at 1 µg/ml + OVCAR-3 cell lysate at 10 µg
    Developed using the ECL technique

    Predicted band size : 55 kDa

References for Anti-FOXN4 antibody (ab98860)

ab98860 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (retina)
Specification retina
Fixative formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate ph 6
Permeabilization Yes - triton and tween
Username

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Verified customer

Submitted Jun 20 2012

DISCOUNT CODE: xx
Expiration date: 14. Juin 2012
I am very pleased to hear you would like to accept our offer and test ab98860 in IHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please ...

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Thank you very much for your interest in ab98860.
To our knowledge, ab98860 has not been tested in immunohistochemistry on paraffin sections (IHC-P). We do not have any indications whether it is suitable or not for IHC-P. If you would like to test...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"