Anti-FOXO1 + FOXO3 + FOXO4 antibody (ab58518)

Overview

  • Product name
    Anti-FOXO1 + FOXO3 + FOXO4 antibody
  • Description
    Rabbit polyclonal to FOXO1 + FOXO3 + FOXO4
  • Tested applications
    Suitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non phosphopeptide derived from human FOXO1/3/4 around the phosphorylation site of threonine 24/32 (S-C-TP-W-P).

  • Positive control
    • IHC-P: Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab58518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • Relevance
    FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors. FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined. FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia. FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.
  • Cellular localization
    FOXO1: Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. FOX3: Cytoplasm, cytosol. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. FOX4: Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation.
  • Database links
  • Alternative names
    • AFX1 antibody
    • FKH1 antibody
    • FKHRL1 antibody
    • FKHRL1P2 antibody
    • Forkhead box O1 antibody
    • Forkhead box O3 antibody
    • Forkhead box O4 antibody
    • FOXO1 antibody
    • FOXO2 antibody
    • FOXO3 antibody
    • FOXO4 antibody
    • MLLT7 antibody
    see all

Images

  • This image shows human breast carcinoma tissue stained with ab58518 at 1/50 dilution. Right hand image; tissue treated with immunising non phosphopeptide. Left hand image; without immunising non phosphopeptide.

References

ab58518 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab58518.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up