Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody (ab58517)


  • Product name
    Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody
    See all FOXO1 + FOXO3 + FOXO4 primary antibodies
  • Description
    Rabbit polyclonal to FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32)
  • Specificity
    ab58517 detects endogenous levels of FOXO1/3/4 only when phosphorylated at threonine 24/32.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human FOXO1/3/4 around the phosphorylation site of threonine 24/32 (S-C-TP-W-P).

  • Positive control
    • IHC-P: human breast carcinoma tissue.



Our Abpromise guarantee covers the use of ab58517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/500 - 1/1000.


  • Relevance
    FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors. FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined. FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia. FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.
  • Cellular localization
    FOXO1: Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. FOX3: Cytoplasm, cytosol. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. FOX4: Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation.
  • Database links
  • Alternative names
    • AFX1 antibody
    • FKH1 antibody
    • FKHRL1 antibody
    • FKHRL1P2 antibody
    • Forkhead box O1 antibody
    • Forkhead box O3 antibody
    • Forkhead box O4 antibody
    • FOXO1 antibody
    • FOXO2 antibody
    • FOXO3 antibody
    • FOXO4 antibody
    • MLLT7 antibody
    see all

Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody images

  • This image shows human breast carcinoma tissue stained with ab58517 at a 1/50 dilution. Right hand image; with immunising phosphopeptide. Left hand image; without immunising phosphopeptide.
  • All lanes : Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody (ab58517)

    Lane 1 : 293 cells
    Lane 2 : 293 cells with blocking phosphopeptide

References for Anti-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody (ab58517)

This product has been referenced in:
  • Urasaki Y  et al. Chronic Uridine Administration Induces Fatty Liver and Pre-Diabetic Conditions in Mice. PLoS One 11:e0146994 (2016). Read more (PubMed: 26789264) »

See 1 Publication for this product

Product Wall

Thank you for your interest in our pFOXO antibodies. None have been tested for reactivity with C. elegans, as far as we know. Here is a list of the antibodies we currently have for pFOXO:

ab47285 - FOXO3A (phospho S253)
ab38501 - FOXO1A...

Read More


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