Overview

  • Product nameAnti-FOXO1A antibody
    See all FOXO1A primary antibodies
  • Description
    Mouse monoclonal to FOXO1A
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein containing the region encoding aa 242-655 of human FKHR expressed in E. coli.

  • Positive control
    • COS cells transfected with human FKHR

Properties

Applications

Our Abpromise guarantee covers the use of ab1102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 2 µg/ml. Predicted molecular weight: 70 kDa.

Target

  • FunctionTranscription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKH 1 antibody
    • FKH1 antibody
    • FKHR antibody
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
    • Forkhead box O1 antibody
    • Forkhead box protein O1 antibody
    • Forkhead box protein O1A antibody
    • Forkhead in rhabdomyosarcoma antibody
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
    • FoxO transcription factor antibody
    • foxo1 antibody
    • FOXO1_HUMAN antibody
    • FOXO1A antibody
    • OTTHUMP00000018301 antibody
    see all

Anti-FOXO1A antibody images

  • ab1102 staining FOXO1A in Human CD4 T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Saponin and blocked with 10% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 3% BSA + 0.1% Saponin) for 1 hour. An Alexa Fluor®647-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References for Anti-FOXO1A antibody (ab1102)

This product has been referenced in:
  • Shioda N  et al. Constitutively active calcineurin mediates delayed neuronal death through Fas-ligand expression via activation of NFAT and FKHR transcriptional activities in mouse brain ischemia. J Neurochem 102:1506-17 (2007). Read more (PubMed: 17662023) »

See 1 Publication for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Human Cell (CD4 T cell)
Specification CD4 T cell
Permeabilization Yes - Saponin (0,1%)
Fixative Paraformaldehyde
Username

Mr. Marco Craveiro

Verified customer

Submitted May 24 2013

Thank you for your enquiry. All the information that we have regarding these two antibodies is located on the online datasheets. We currently do not have information about specificity to O1A/o1a and other FOXO/foxo family members. If you have any more ...

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Thank you for your patience and your detailed protocol. We are very sorry to hear that you are having problem with this antibody. We have searched our database and found that this is a popular selling product and your feedback is the first we have ...

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Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet and we unfortunately do not have a picture of a blot at this time. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"