Overview

  • Product nameAnti-FOXO1A antibody
    See all FOXO1A primary antibodies
  • Description
    Rabbit polyclonal to FOXO1A
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 329.

  • Positive control
    • Human breast carcinoma tissue, extracts from HeLa cells, treated with Serum (20%, 15mins).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab61760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 70 kDa.

Target

  • FunctionTranscription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKH 1 antibody
    • FKH1 antibody
    • FKHR antibody
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
    • Forkhead box O1 antibody
    • Forkhead box protein O1 antibody
    • Forkhead box protein O1A antibody
    • Forkhead in rhabdomyosarcoma antibody
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
    • FoxO transcription factor antibody
    • foxo1 antibody
    • FOXO1_HUMAN antibody
    • FOXO1A antibody
    • OTTHUMP00000018301 antibody
    see all

Anti-FOXO1A antibody images

  • Human breast carcinoma tissue stained with ab61760 at 1/50 dilution, with and without the immunising peptide.
  • All lanes : Anti-FOXO1A antibody (ab61760) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells, treated with Serum (20%, 15mins).
    Lane 2 : Extracts from HeLa cells, treated with Serum (20%, 15mins), and the immunising peptide.


    Predicted band size : 70 kDa
    Observed band size : 70 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-FOXO1A antibody (ab61760)

This product has been referenced in:
  • Huang H  et al. Rho-kinase regulates energy balance by targeting hypothalamic leptin receptor signaling. Nat Neurosci 15:1391-8 (2012). Read more (PubMed: 22941110) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"