The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/3000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
Use at 1-10 µg/mg of lysate.
Use at an assay dependent concentration.
Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
Involvement in disease
Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
Contains 1 fork-head DNA-binding domain.
Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling FOXO1A with ab70382 at 1/500 (2µg/ml). Detection: DAB.
Western blot - FOXO1A antibody (ab70382)
Lane 1 : Anti-FOXO1A antibody (ab70382) at 0.3 µg/ml Lane 2 : Anti-FOXO1A antibody (ab70382) at 0.1 µg/ml
Lane 1 : Hek293 whole cell lysate Lane 2 : Hek293 whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique
Predicted band size : 70 kDa Observed band size : 70 kDa Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - FOXO1A antibody (ab70382)
Detection of Human FOXO1A by Immunoprecipitation. ab70382 used at 5µg/0.5mg Hek293 whole cell lysate for IP and 0.1µg/ml for WB. IP was controlled using a rabbit antibody to an unrelated protein. Detection: Chemiluminescence with exposure times of less than 2 minutes.