Overview

  • Product nameAnti-FOXO1A antibody
    See all FOXO1A primary antibodies
  • Description
    Rabbit polyclonal to FOXO1A
  • Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide mapping to a region between residues 600 and the C-terminus (residue 652) of human FOXO1A (Swiss-Prot entry Q12778)

  • Positive control
    • HEK293 whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP Use at 1-10 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/500.

Target

  • FunctionTranscription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKH 1 antibody
    • FKH1 antibody
    • FKHR antibody
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
    • Forkhead box O1 antibody
    • Forkhead box protein O1 antibody
    • Forkhead box protein O1A antibody
    • Forkhead in rhabdomyosarcoma antibody
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
    • FoxO transcription factor antibody
    • foxo1 antibody
    • FOXO1_HUMAN antibody
    • FOXO1A antibody
    • OTTHUMP00000018301 antibody
    see all

Anti-FOXO1A antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling FOXO1A with ab70382 at 1/500 (2µg/ml). Detection: DAB.
  • Lane 1 : Anti-FOXO1A antibody (ab70382) at 0.3 µg/ml
    Lane 2 : Anti-FOXO1A antibody (ab70382) at 0.1 µg/ml

    Lane 1 : Hek293 whole cell lysate
    Lane 2 : Hek293 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human FOXO1A by Immunoprecipitation. ab70382 used at 5µg/0.5mg Hek293 whole cell lysate for IP and 0.1µg/ml for WB. IP was controlled using a rabbit antibody to an unrelated protein. Detection: Chemiluminescence with exposure times of less than 2 minutes.

References for Anti-FOXO1A antibody (ab70382)

This product has been referenced in:
  • Ochodnicka-Mackovicova K  et al. The DNA Damage Response Regulates RAG1/2 Expression in Pre-B Cells through ATM-FOXO1 Signaling. J Immunol 197:2918-29 (2016). Read more (PubMed: 27559048) »
  • Timblin GA & Schlissel MS Ebf1 and c-Myb repress rag transcription downstream of Stat5 during early B cell development. J Immunol 191:4676-87 (2013). Read more (PubMed: 24068669) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Pig Cell lysate - whole cell (Heart)
Gel Running Conditions Non-reduced Denaturing (8% gel)
Loading amount 30 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Jul 24 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Human Cell (human fibroblast cell)
Specification human fibroblast cell
Permeabilization Yes - Triton X100
Fixative Formaldehyde
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Submitted Jan 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (human fibroblst)
Specification human fibroblst
Total protein in input 500 µg
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Submitted Sep 23 2013

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Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (human fibroblast)
Specification human fibroblast
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"