Recombinant
RabMAb

Anti-FOXO1A antibody [EP927Y] (ab52857)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab52857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/90.
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
    • Tissue specificity
      Ubiquitous.
    • Involvement in disease
      Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
    • Sequence similarities
      Contains 1 fork-head DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FKH 1 antibody
      • FKH1 antibody
      • FKHR antibody
      • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
      • Forkhead box O1 antibody
      • Forkhead box protein O1 antibody
      • Forkhead box protein O1A antibody
      • Forkhead in rhabdomyosarcoma antibody
      • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
      • FoxO transcription factor antibody
      • foxo1 antibody
      • FOXO1_HUMAN antibody
      • FOXO1A antibody
      • OTTHUMP00000018301 antibody
      see all

    Images

    • Anti-FOXO1A antibody [EP927Y] (ab52857) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 70 kDa
      Observed band size : 70 kDa
    • ab52857 at 1/250 dilution staining FOXO1A in human bladder carcinoma by Immunohistochemisty, Paraffin embedded tissue.
    • ab52857 at 1/100 dilution staining FOXO1A in HeLa cells by Immunocytochemistry.
    • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling FOXO1A (red) with ab52857 at a 1/90 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    References

    This product has been referenced in:
    • Yang N  et al. Propofol inhibits lung cancer cell viability and induces cell apoptosis by upregulating microRNA-486 expression. Braz J Med Biol Res 50:e5794 (2017). WB . Read more (PubMed: 28076456) »
    • Hu L  et al. ?Np63a is a common inhibitory target in oncogenic PI3K/Ras/Her2-induced cell motility and tumor metastasis. Proc Natl Acad Sci U S A 114:E3964-E3973 (2017). Read more (PubMed: 28468801) »

    See all 17 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Liver Extract)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-Glycin)
    Loading amount
    20 µg
    Specification
    Liver Extract
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 25 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100 µg
    Specification
    human cell
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 02 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
    Permeabilization
    Yes - 0.025% Triton x-100
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

    Application
    Western blot
    Loading amount
    36 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Rat Tissue lysate - whole (Bone - Tibia)
    Specification
    Bone - Tibia
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mr. Helder Fonseca

    Verified customer

    Submitted Sep 25 2014

    Application
    Western blot
    Loading amount
    100000 cells
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rat Cell lysate - whole cell (rat vascular smooth musle cells)
    Specification
    rat vascular smooth musle cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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