Overview

Applications

Our Abpromise guarantee covers the use of ab52857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500.
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionTranscription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
    • Tissue specificityUbiquitous.
    • Involvement in diseaseDefects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
    • Sequence similaritiesContains 1 fork-head DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FKH 1 antibody
      • FKH1 antibody
      • FKHR antibody
      • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
      • Forkhead box O1 antibody
      • Forkhead box protein O1 antibody
      • Forkhead box protein O1A antibody
      • Forkhead in rhabdomyosarcoma antibody
      • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
      • FoxO transcription factor antibody
      • foxo1 antibody
      • FOXO1_HUMAN antibody
      • FOXO1A antibody
      • OTTHUMP00000018301 antibody
      see all

    Anti-FOXO1A antibody [EP927Y] images

    • Anti-FOXO1A antibody [EP927Y] (ab52857) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 70 kDa
      Observed band size : 70 kDa
    • ab52857 at 1/250 dilution staining FOXO1A in human bladder carcinoma by Immunohistochemisty, Paraffin embedded tissue.
    • ab52857 at 1/100 dilution staining FOXO1A in HeLa cells by Immunocytochemistry.

    References for Anti-FOXO1A antibody [EP927Y] (ab52857)

    This product has been referenced in:
    • Pomiès P  et al. Involvement of the FoxO1/MuRF1/Atrogin-1 Signaling Pathway in the Oxidative Stress-Induced Atrophy of Cultured Chronic Obstructive Pulmonary Disease Myotubes. PLoS One 11:e0160092 (2016). Read more (PubMed: 27526027) »
    • Shao D  et al. A functional interaction between Hippo-YAP signalling and FoxO1 mediates the oxidative stress response. Nat Commun 5:3315 (2014). Read more (PubMed: 24525530) »

    See all 14 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Lung)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
    Permeabilization Yes - 0.025% Triton x-100
    Specification Lung
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

    Application Western blot
    Loading amount 36 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Rat Tissue lysate - whole (Bone - Tibia)
    Specification Bone - Tibia
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mr. Helder Fonseca

    Verified customer

    Submitted Sep 25 2014

    Application Western blot
    Loading amount 100000 cells
    Gel Running Conditions Reduced Denaturing
    Sample Rat Cell lysate - whole cell (rat vascular smooth musle cells)
    Specification rat vascular smooth musle cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"