Anti-FOXO1A (phospho S256) antibody (ab131339)
Key features and details
- Rabbit polyclonal to FOXO1A (phospho S256)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOXO1A (phospho S256) antibody
See all FOXO1A primary antibodies -
Description
Rabbit polyclonal to FOXO1A (phospho S256) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human FOXO1A conjugated to keyhole limpet haemocyanin. Synthetic peptide corresponding to the sequence around phosphorylation site of serine 256 (
A-A-S(p)-M-D
) of Human FOXO1A (NP_002006.2).
Sequence:A-A-S(p)-M-D
Database link: Q12778 -
Positive control
- MCF7 cells, 293 cell extracts, Human breast carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.88% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131339 is purified by affinity-chromatography using an epitode-specific phosphopetide. Non-phoso specific antibodies are removed by chromatography using a non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/500 - 1/1000. Detects a band of approximately 78-82 kDa (predicted molecular weight: 69 kDa). Incubate membrane with diluted antibody in 5% non fatmilk, 1X TBS, 0.1% Tween 20 at 4 C, with gentle shaking, overnight.
|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF | (1) |
1/100 - 1/200.
|
Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 78-82 kDa (predicted molecular weight: 69 kDa). Incubate membrane with diluted antibody in 5% non fatmilk, 1X TBS, 0.1% Tween 20 at 4 C, with gentle shaking, overnight. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Post-translational
modificationsPhosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2308 Human
- Omim: 136533 Human
- SwissProt: Q12778 Human
- Unigene: 370666 Human
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Alternative names
- FKH 1 antibody
- FKH1 antibody
- FKHR antibody
see all
Images
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Immunofluorescent analysis of formalin-fixed, paraffin-embedded human breast tissue with Anti-FOXO1A at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.
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All lanes : Anti-FOXO1A (phospho S256) antibody (ab131339) at 1/500 dilution
Lane 1 : 293 cell extracts
Lane 2 : 293 cell extracts treated with serum
Predicted band size: 69 kDa -
Immunofluorescence analysis of methanol-fixed MCF7 cells using ab131339 at a 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab131339 at a 1/50 dilution, in the absence (left) and presence (right) of the blocking peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab131339 has been referenced in 35 publications.
- Peng M et al. Programmed death-ligand 1 signaling and expression are reversible by lycopene via PI3K/AKT and Raf/MEK/ERK pathways in tongue squamous cell carcinoma. Genes Nutr 17:3 (2022). PubMed: 35164673
- Wu B et al. Aerobic exercise promotes the expression of ATGL and attenuates inflammation to improve hepatic steatosis via lncRNA SRA. Sci Rep 12:5370 (2022). PubMed: 35354841
- Dai L et al. Propofol inhibits the malignant development of osteosarcoma U2OS cells via AMPK/FΟΧO1-mediated autophagy. Oncol Lett 24:310 (2022). PubMed: 35949604
- Ren HZ et al. FOXO1 Alleviates Liver Ischemia-reperfusion Injury by Regulating the Th17/Treg Ratio through the AKT/Stat3/FOXO1 Pathway. J Clin Transl Hepatol 10:1138-1147 (2022). PubMed: 36381102
- Zhao HY et al. L-carnitine treatment attenuates renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction. Korean J Intern Med 36:S180-S195 (2021). PubMed: 32942841