Anti-FOXO1A (phospho S319) antibody (ab47326)

Overview

  • Product name
    Anti-FOXO1A (phospho S319) antibody
    See all FOXO1A primary antibodies
  • Description
    Rabbit polyclonal to FOXO1A (phospho S319)
  • Specificity
    This antibody detects endogenous levels of FOXO1A only when phosphorylated at serine 319.(Human: Ser319; Mouse: Ser316; Rat: Ser313)
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 319 (T-S-SP-N-A)

  • Positive control
    • human breast carcinoma, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab47326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
IHC-P
ELISA
  • Application notes
    ELISA: 1/10000.
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
    • Tissue specificity
      Ubiquitous.
    • Involvement in disease
      Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
    • Sequence similarities
      Contains 1 fork-head DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FKH 1 antibody
      • FKH1 antibody
      • FKHR antibody
      • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
      • Forkhead box O1 antibody
      • Forkhead box protein O1 antibody
      • Forkhead box protein O1A antibody
      • Forkhead in rhabdomyosarcoma antibody
      • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
      • FoxO transcription factor antibody
      • foxo1 antibody
      • FOXO1_HUMAN antibody
      • FOXO1A antibody
      • OTTHUMP00000018301 antibody
      see all

    Images

    • ab47326 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
    • All lanes : Anti-FOXO1A (phospho S319) antibody (ab47326)

      Lane 1 : extracts from EGF treated HeLa cells
      Lane 2 : extracts from HeLa cells


      Predicted band size : 70 kDa
    • ICC/IF image of ab47326 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47326, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    ab47326 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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