Overview

  • Product nameAnti-FOXO3A antibody
    See all FOXO3A primary antibodies
  • Description
    Rabbit polyclonal to FOXO3A
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 250 and 300 of human FOXO3A. (Swiss-Prot O43524)

  • Positive control
    • Whole cell lysate from MCF7 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/15000. Detects a band of approximately 85 kDa (predicted molecular weight: 71 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • FunctionTranscriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseNote=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1.
  • Cellular localizationCytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF6q21 antibody
    • AF6q21 protein antibody
    • DKFZp781A0677 antibody
    • FKHR2 antibody
    • FKHRL 1 antibody
    • FKHRL1 antibody
    • FKHRL1P2 antibody
    • Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody
    • Forkhead box O3 antibody
    • Forkhead box O3A antibody
    • Forkhead box protein O3 antibody
    • Forkhead box protein O3A antibody
    • Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
    • Forkhead homolog (rhabdomyosarcoma) like 1 antibody
    • Forkhead in rhabdomyosarcoma like 1 antibody
    • Forkhead in rhabdomyosarcoma-like 1 antibody
    • FOX O3A antibody
    • FOXO2 antibody
    • foxo3 antibody
    • FOXO3_HUMAN antibody
    • FOXO3A antibody
    • MGC12739 antibody
    • MGC31925 antibody
    see all

Anti-FOXO3A antibody images

  • Lane 1 : MCF7 Cell Lysate Immunoprecipitated with ab70314 at 6ug/mg.

    Lane 2: MCF7 Cell Lysate Immunoprecipitated with ab70315 6ug/mg

    Lane 3:MCF7 Cell Lysate Immunoprecipitated with Control IgG at 6ug/mg

     

    Western Blot carried out using ab70314 at 1ug/ml

    Decction: ECL for 3 minutes.

     

  • All lanes : Anti-FOXO3A antibody (ab70314) at 0.4 µg/ml

    Lane 1 : 293T cell Lysate
    Lane 2 : Jurkat cell Lysate
    Lane 3 : Mouse TCMK cell Lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 71 kDa


    Exposure time : 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling FOXO3A with ab70314 at 1/500 (2µg/ml). Detection: DAB.

References for Anti-FOXO3A antibody (ab70314)

ab70314 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Fly heads)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification Fly heads
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Aug 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"