Anti-FOXO3A antibody (ab70314)
Key features and details
- Rabbit polyclonal to FOXO3A
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-FOXO3A antibody
See all FOXO3A primary antibodies -
Description
Rabbit polyclonal to FOXO3A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to a region between residues 250 and 300 of human FOXO3A. (Swiss-Prot O43524)
-
Positive control
- Whole cell lysate from MCF7 cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000 - 1/15000. Detects a band of approximately 85 kDa (predicted molecular weight: 71 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
|
IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/5000 - 1/15000. Detects a band of approximately 85 kDa (predicted molecular weight: 71 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Post-translational
modificationsIn the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. - Information by UniProt
-
Database links
- Entrez Gene: 535530 Cow
- Entrez Gene: 481954 Dog
- Entrez Gene: 100066558 Horse
- Entrez Gene: 2309 Human
- Entrez Gene: 56484 Mouse
- Entrez Gene: 733621 Pig
- Omim: 602681 Human
- SwissProt: O43524 Human
see all -
Alternative names
- AF6q21 antibody
- AF6q21 protein antibody
- DKFZp781A0677 antibody
see all
Images
-
All lanes : Anti-FOXO3A antibody (ab70314) at 0.4 µg/ml
Lane 1 : 293T cell Lysate
Lane 2 : Jurkat cell Lysate
Lane 3 : Mouse TCMK cell Lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 3 minutes -
Lane 1 : MCF7 Cell Lysate Immunoprecipitated with ab70314 at 6ug/mg.
Lane 2: MCF7 Cell Lysate Immunoprecipitated with ab70315 6ug/mg
Lane 3:MCF7 Cell Lysate Immunoprecipitated with Control IgG at 6ug/mg
Western Blot carried out using ab70314 at 1ug/ml
Decction: ECL for 3 minutes.
All lanes : Anti-FOXO3A antibody (ab70314) at 1 µg/ml
Lane 1 : MCF7 Cell Lysate Immunoprecipitated with ab70314 at 6ug/mg
Lane 2 : MCF7 Cell Lysate Immunoprecipitated with ab70315 6ug/mg
Lane 3 : MCF7 Cell Lysate Immunoprecipitated with Control IgG at 6ug/mg
Developed using the ECL technique.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling FOXO3A with ab70314 at 1/500 (2µg/ml). Detection: DAB.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab70314 has been referenced in 1 publication.
- Wang Y et al. miR-155 promotes fibroblast-like synoviocyte proliferation and inflammatory cytokine secretion in rheumatoid arthritis by targeting FOXO3a. Exp Ther Med 19:1288-1296 (2020). PubMed: 32010301