Recombinant Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1951(2)] to FOXO3A (phospho S253)
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]
See all FOXO3A primary antibodies -
Description
Rabbit monoclonal [EPR1951(2)] to FOXO3A (phospho S253) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Dot blotmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF-7 cell lysate treated with IGF; Human endometrial carcinoma tissue; Human pancreas tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1951(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Dot blot |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 71 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Dot blot
Use at an assay dependent concentration. |
Target
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Function
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Post-translational
modificationsIn the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. - Information by UniProt
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Database links
- Entrez Gene: 2309 Human
- Entrez Gene: 56484 Mouse
- Entrez Gene: 294515 Rat
- Omim: 602681 Human
- SwissProt: O43524 Human
- SwissProt: Q9WVH4 Mouse
- Unigene: 220950 Human
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Alternative names
- AF6q21 antibody
- AF6q21 protein antibody
- DKFZp781A0677 antibody
see all
Images
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All lanes : Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786) at 1/5000 dilution
Lane 1 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with IGF-1. Whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human normal pancreas tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Dot Blot analysis of Lane 1: FOXO3A (pS253) phospho peptide and Lane 2: FOXO3A non-phospho peptide with ab154786 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer and concentration. ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab154786 has been referenced in 40 publications.
- Sun R & Zheng Q AKT/foxo3a signal pathway mediates the protective mechanism of resveratrol on renal interstitial fibrosis and oxidative stress in rats with unilateral ureteral obstruction. Am J Transl Res 14:1788-1795 (2022). PubMed: 35422955
- Xu J et al. Inhibition of the cardiac fibroblast-enriched histone methyltransferase Dot1L prevents cardiac fibrosis and cardiac dysfunction. Cell Biosci 12:134 (2022). PubMed: 35986422
- Yao F & Zhu Q α-Cyperone Protects Cardiomyocytes against Oxygen-Glucose Deprivation-Induced Inflammation and Oxidative Stress by Akt/FOXO3a/NF-κB Pathway. Dis Markers 2022:8205707 (2022). PubMed: 36072899
- Qiu T et al. Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1. Cancers (Basel) 14:N/A (2022). PubMed: 36139584
- Du X et al. Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC. J Transl Med 20:507 (2022). PubMed: 36335371