• Product nameAnti-FOXO4 antibody
    See all FOXO4 primary antibodies
  • Description
    Rabbit polyclonal to FOXO4
  • SpecificityThis antibody is specific for FOXO4.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthesized non-phosphopeptide derived from human FOXO4 around the phosphorylation site of serine 197.

  • Positive control
    • Extracts from 293 cells and human lung carcinoma tissue


Associated products


Our Abpromise guarantee covers the use of ab47396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.


  • FunctionTranscription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
  • Involvement in diseaseNote=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similaritiesContains 1 fork-head DNA-binding domain.
  • Post-translational
    Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
    Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
    Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
  • Cellular localizationCytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AFX antibody
    • AFX1 antibody
    • Afxh antibody
    • ALL1-fused gene from X chromosome antibody
    • Fork head domain transcription factor AFX1 antibody
    • Forkhead box O4 antibody
    • Forkhead box protein O4 antibody
    • FOXO 4 antibody
    • Foxo4 antibody
    • FOXO4_HUMAN antibody
    • MGC117660 antibody
    • MGC120490 antibody
    • Mixed lineage leukemia, translocated to, 7 antibody
    • MLLT7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
    • RGD1561201 antibody
    see all

Anti-FOXO4 antibody images

  • All lanes : Anti-FOXO4 antibody (ab47396) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with serum
    Lane 2 : Extracts from 293 cells treated with serum and peptide

    Predicted band size : 54 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab47396 at a 1/50 dilution.

    Left image : Un-treated

    Right image : Treated with peptide

References for Anti-FOXO4 antibody (ab47396)

This product has been referenced in:
  • Iwata J  et al. Transforming growth factor-beta regulates basal transcriptional regulatory machinery to control cell proliferation and differentiation in cranial neural crest-derived osteoprogenitor cells. J Biol Chem 285:4975-82 (2010). WB ; Mouse . Read more (PubMed: 19959467) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T transfected with epitope-tagged FOXO4)
Loading amount 20 µg
Specification 293T transfected with epitope-tagged FOXO4
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

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Submitted Jul 02 2008