Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXO4
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human FOXO4 around the phosphorylation site of threonine 451

  • Positive control
    • K562 cells, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab63254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function
    Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
  • Tissue specificity
    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
  • Involvement in disease
    Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
    Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
    Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
  • Cellular localization
    Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AFX antibody
    • AFX1 antibody
    • Afxh antibody
    • ALL1-fused gene from X chromosome antibody
    • Fork head domain transcription factor AFX1 antibody
    • Forkhead box O4 antibody
    • Forkhead box protein O4 antibody
    • FOXO 4 antibody
    • Foxo4 antibody
    • FOXO4_HUMAN antibody
    • MGC117660 antibody
    • MGC120490 antibody
    • Mixed lineage leukemia, translocated to, 7 antibody
    • MLLT7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
    • RGD1561201 antibody
    see all

Anti-FOXO4 antibody images

  • All lanes : Anti-FOXO4 antibody (ab63254) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from K562 cells, with immunizing peptide


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Immunofluorescence analysis of FOXO4 expression in HeLa cells, using 1/500 ab63254. Left: untreated sample. Right: sample treated with immunising peptide.
  • Ab63254 staining Human normal pancreas. Staining is localised to the nucleus and cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-FOXO4 antibody (ab63254)

This product has been referenced in:
  • Liu X  et al. MicroRNA-499-5p promotes cellular invasion and tumor metastasis in colorectal cancer by targeting FOXO4 and PDCD4. Carcinogenesis 32:1798-805 (2011). WB ; Human . Read more (PubMed: 21934092) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Antigen retrieval step
Other
Sample
Mouse Tissue sections (chondrocytes)
Specification
chondrocytes
Permeabilization
No
Fixative
zinc buffered formalin
Username

Abcam user community

Verified customer

Submitted Mar 17 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (fibroblast)
Specification
fibroblast
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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