Recombinant
RabMAb

Anti-FOXO4 antibody [EPR2150(3)] (ab126757)

Overview

  • Product name
    Anti-FOXO4 antibody [EPR2150(3)]
    See all FOXO4 primary antibodies
  • Description
    Rabbit monoclonal [EPR2150(3)] to FOXO4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human FOXO4.

  • Positive control
    • HA-FOXO4 transfected 293T cell lysate; HA-FOXO4.A3 (T32, S197 and S262 mutated to A) transfected 293T cell lysate; Human placenta tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    This antibody was developed as part of a collaboration between Epitomics, the University of Utrecht, Netherlands and the lab of Tobias Dansen.

    Produced under U.S. Patent No. 5,675,063.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab126757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.)
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
    • Tissue specificity
      Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
    • Involvement in disease
      Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
    • Sequence similarities
      Contains 1 fork-head DNA-binding domain.
    • Post-translational
      modifications
      Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
      Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
      Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
    • Cellular localization
      Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AFX antibody
      • AFX1 antibody
      • Afxh antibody
      • ALL1-fused gene from X chromosome antibody
      • Fork head domain transcription factor AFX1 antibody
      • Forkhead box O4 antibody
      • Forkhead box protein O4 antibody
      • FOXO 4 antibody
      • Foxo4 antibody
      • FOXO4_HUMAN antibody
      • MGC117660 antibody
      • MGC120490 antibody
      • Mixed lineage leukemia, translocated to, 7 antibody
      • MLLT7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
      • RGD1561201 antibody
      see all

    Images

    • All lanes : Anti-FOXO4 antibody [EPR2150(3)] (ab126757) at 1/10000 dilution

      Lane 1 : HA-FOXO4 transfected 293T cell lysate
      Lane 2 : HA-FOXO4.A3 (T32, S197 and S262 mutated to A) transfected 293T cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 53 kDa
    • ab126757, at 1/100 dilution, staining FOXO4 in paraffin-embedded Human placenta tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126757 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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