Anti-FOXO4 (phospho S193) antibody (ab2560)

Overview

  • Product name
    Anti-FOXO4 (phospho S193) antibody
    See all FOXO4 primary antibodies
  • Description
    Rabbit polyclonal to FOXO4 (phospho S193)
  • Specificity
    This antibody recognizes the phosphorylated Ser193 of AFX. It does not react with non-phosphorylated AFX.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Hamster, Human
  • Immunogen

    Available on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab2560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 54 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
    • Tissue specificity
      Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
    • Involvement in disease
      Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
    • Sequence similarities
      Contains 1 fork-head DNA-binding domain.
    • Post-translational
      modifications
      Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
      Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
      Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
    • Cellular localization
      Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AFX antibody
      • AFX1 antibody
      • Afxh antibody
      • ALL1-fused gene from X chromosome antibody
      • Fork head domain transcription factor AFX1 antibody
      • Forkhead box O4 antibody
      • Forkhead box protein O4 antibody
      • FOXO 4 antibody
      • Foxo4 antibody
      • FOXO4_HUMAN antibody
      • MGC117660 antibody
      • MGC120490 antibody
      • Mixed lineage leukemia, translocated to, 7 antibody
      • MLLT7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
      • RGD1561201 antibody
      see all

    Images

    • ICC/IF image of ab2560 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2560, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    ab2560 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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