Anti-FOXO4 (phospho T451) antibody (ab79188)

Overview

  • Product name
    Anti-FOXO4 (phospho T451) antibody
    See all FOXO4 primary antibodies
  • Description
    Rabbit polyclonal to FOXO4 (phospho T451)
  • Specificity
    ab79188 detects endogenous levels of FOXO4 only when phosphorylated at threonine 451 (Human: Thr451; Mouse: Thr452).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human FOXO4 around the phosphorylation site of threonine 451 (L-G-TP-P-V).

  • Positive control
    • Extracts from HUVEC cells treated with EGF (200ng/ml, 5mins).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab79188 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.
ELISA 1/10000.

Target

  • Function
    Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
  • Tissue specificity
    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
  • Involvement in disease
    Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
    Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
    Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
  • Cellular localization
    Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AFX antibody
    • AFX1 antibody
    • Afxh antibody
    • ALL1-fused gene from X chromosome antibody
    • Fork head domain transcription factor AFX1 antibody
    • Forkhead box O4 antibody
    • Forkhead box protein O4 antibody
    • FOXO 4 antibody
    • Foxo4 antibody
    • FOXO4_HUMAN antibody
    • MGC117660 antibody
    • MGC120490 antibody
    • Mixed lineage leukemia, translocated to, 7 antibody
    • MLLT7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
    • RGD1561201 antibody
    see all

Images

  • All lanes : Anti-FOXO4 (phospho T451) antibody (ab79188) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells treated with EGF (200ng/ml, 5mins)
    Lane 2 : Extracts from HUVEC cells treated with EGF (200ng/ml, 5mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 54 kDa
    Observed band size : 54 kDa

References

This product has been referenced in:
  • Salim H  et al. miRNA-214 modulates radiotherapy response of non-small cell lung cancer cells through regulation of p38MAPK, apoptosis and senescence. Br J Cancer 107:1361-73 (2012). Read more (PubMed: 22929890) »

See 1 Publication for this product

Customer reviews and Q&As

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Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
60 µg
Specification
heart
Gel Running Conditions
Non-reduced Denaturing (10)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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Submitted Feb 09 2012

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