Overview

  • Product nameAnti-Foxo6 antibody
    See all Foxo6 primary antibodies
  • Description
    Rabbit polyclonal to Foxo6
  • SpecificityThis antibody recognizes Foxo6
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide: RAVSMDNGAK FLRIKGKASK KKQLHLPERS PDDSPPGAPV PGPLSASAKW, corresponding to a region within N terminal amino acids 181-230 of Mouse Foxo6

  • Positive control
    • WB: SP2/0 cell lysate. ICC/IF: MEF1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab48730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceMurine Foxo6 is a member of the murine forkhead family of transcription factors. This family consists of over 30 members, the vast majority of which is important in embryonic development. These forkhead transcription factors may play a role in maintenance and survival of developing and adult neurons.
  • Cellular localizationCytoplasmic and Nuclear. When phosphorylated, translocated from nucleus to cytoplasm. High nuclear localization after stimulation with growth factors.
  • Database links
  • Alternative names
    • Forkhead box protein O6 antibody

Anti-Foxo6 antibody images

  • Anti-Foxo6 antibody (ab48730) at 1.25 µg/ml + SP2/0 lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab48730 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48730, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Foxo6 antibody (ab48730)

ab48730 has not yet been referenced specifically in any publications.

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