Overview

  • Product nameAnti-FOXP1 antibody [JC12]
    See all FOXP1 primary antibodies
  • Description
    Mouse monoclonal [JC12] to FOXP1
  • SpecificityThis antibody does not recognise closely related molecules FOXP2, FOXP3 or FOXP4.
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Zebra finch
  • Immunogen

    Full length native protein (purified) of Mouse FOXP1. JC12 is thought to be a mouse autoantibody.

  • Positive control
    • BCBL1 human cell line or Mutul human cell line. Human tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab32010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB 1/500. Predicted molecular weight: 75 kDa.

Target

  • FunctionTranscriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development.
  • Involvement in diseaseNote=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
    Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors.
  • Sequence similaritiesContains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • DomainThe leucine-zipper is required for dimerization and transcriptional repression.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12CC4 antibody
    • FLJ23741 antibody
    • Fork head related protein like B antibody
    • Forkhead box P1 antibody
    • Forkhead box protein P1 antibody
    • FOX P1 antibody
    • FOXP 1 antibody
    • foxp1 antibody
    • FOXP1_HUMAN antibody
    • Glutamine rich factor 1 antibody
    • hFKH1B antibody
    • HSPC215 antibody
    • MGC12942 antibody
    • MGC88572 antibody
    • MGC99551 antibody
    • QRF 1 antibody
    • QRF1 antibody
    see all

Anti-FOXP1 antibody [JC12] images

  • All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 1/500 dilution

    Lane 1 : BCBL1 human cell line
    Lane 2 : Mutul human cell line


    Predicted band size : 75 kDa
    Observed band size : 60,65,80 kDa (why is the actual band size different from the predicted?)
    This antibody recognizes a long form and two short forms of human FOXP1 as described in Blood. 2008. Mar 1; 111(5): 2816-24.

    Image kindly provided by H. Hu.
  • All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 10 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HEK293 Whole Cell Lysate Overexpressing FOXP1

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution

    Predicted band size : 75 kDa
    Observed band size : 80,100 kDa (why is the actual band size different from the predicted?)

References for Anti-FOXP1 antibody [JC12] (ab32010)

This product has been referenced in:
  • Lu TX  et al. Epstein-Barr virus positive diffuse large B-cell lymphoma predict poor outcome, regardless of the age. Sci Rep 5:12168 (2015). IHC ; Human . Read more (PubMed: 26202875) »
  • Rouhigharabaei L  et al. Non-IG aberrations of FOXP1 in B-cell malignancies lead to an aberrant expression of N-truncated isoforms of FOXP1. PLoS One 9:e85851 (2014). WB ; Human . Read more (PubMed: 24416450) »

See all 17 Publications for this product

Product Wall

Application Flow Cytometry
Sample Human Cell (Differentiated hNSCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Undifferentiated Stem Cells (white)
Specification Differentiated hNSCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
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Verified customer

Submitted Sep 09 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (5% Tris-Glycine)
Sample Zebra finch Cell lysate - whole cell (Hek overexpressing zebra finch FoxP1)
Specification Hek overexpressing zebra finch FoxP1
Blocking step Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Aug 14 2013

Thanks for your call today and for letting us know about the trouble with these antibodies. As we discussed, I am sending two free of charge replacements that should arrive tomorrow on the order ***. Please keep me updated on how these work in ...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain tissue)
Specification brain tissue
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization Yes - PBS-T
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Submitted May 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 60 µg
Specification 293T
Treatment transfected with EGFP FOXP 1
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Apr 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"