Overview

  • Product name
    Anti-FOXP3 antibody [2A11G9]
    See all FOXP3 primary antibodies
  • Description
    Mouse monoclonal [2A11G9] to FOXP3
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, ELISAmore details
    Unsuitable for: IHC-Fr or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Ni-NTA purified truncated recombinant Foxp3 expressed in E. Coli strain BL21 (DE3).

  • Positive control
    • HEK293 cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab36607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
WB 1/500 - 1/2000. Detects a band of approximately 50-53 kDa (predicted molecular weight: 47 kDa).
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA 1/10000.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.
  • Target

    • Function
      Probable transcription factor. Plays a critical role in the control of immune response.
    • Involvement in disease
      Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
    • Sequence similarities
      Contains 1 C2H2-type zinc finger.
      Contains 1 fork-head DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AIID antibody
      • DIETER antibody
      • Forkhead box P3 antibody
      • Forkhead box protein P3 antibody
      • FOXP3 antibody
      • FOXP3_HUMAN antibody
      • FOXP3delta7 antibody
      • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
      • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
      • IPEX antibody
      • JM2 antibody
      • MGC141961 antibody
      • MGC141963 antibody
      • OTTHUMP00000025832 antibody
      • OTTHUMP00000025833 antibody
      • OTTHUMP00000226737 antibody
      • PIDX antibody
      • Scurfin antibody
      • XPID antibody
      see all

    Images

    • All lanes : Anti-FOXP3 antibody [2A11G9] (ab36607) at 1/1000 dilution

      Lane 1 : Human truncated Foxp3 recombinant protein
      Lane 2 : HEK293 cell extract

      Lysates/proteins at 2 µg/ml per lane.


      Predicted band size : 47 kDa
      Observed band size : ~50-53 kDa (why is the actual band size different from the predicted?)
    • ab36607 at 1/1000 dilution staining FOXP3 in human PANC1 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. The primary antibody show green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 Phalloidin.

    • Overlay histogram showing Jurkat cells stained with ab36607 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36607, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References

    This product has been referenced in:
    • Ji H  et al. Sex-specific T-cell regulation of angiotensin II-dependent hypertension. Hypertension 64:573-82 (2014). Read more (PubMed: 24935938) »
    • Imam S  et al. Diabetes-associated dry eye syndrome in a new humanized transgenic model of type 1 diabetes. Mol Vis 19:1259-67 (2013). ICC/IF ; Mouse . Read more (PubMed: 23805032) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Unfortunately, ab36607 is not recommended for IHC applications (IHC-Fr or IHC-P).

    For Mouse-on-Mouse (MOM) staining with other mouse-host antibodies, we can recommend the following IHC detection kit: ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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