The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Predicted molecular weight: 47 kDa.
Use a concentration of 2 - 10 µg/ml. Optimal concentration for peripheral blood is 3 µg/ml.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionProbable transcription factor. Plays a critical role in the control of immune response.
Involvement in diseaseDefects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
ab99873 performing intracellular staining of human peripheral blood cells (gated on CD4+ cells).
References for Anti-FOXP3 antibody [3G3] (ab99873)
This product has been referenced in:
Gavin MA et al. Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A103:6659-64 (2006).
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