• Product nameAnti-FOXP3 antibody
    See all FOXP3 primary antibodies
  • Description
    Rabbit polyclonal to FOXP3
  • SpecificityThis antibody recognises a single clean band representing FOXP3 on HEK293 cell lysate overexpressing human FOXP3. This band is blocked by the immunising peptide and is not present in the control HEK293 cell lysate.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human FOXP3.


  • Positive control
    • This antibody gave a positive signal in Hek 293 lysate over expressing human FOXP3



Our Abpromise guarantee covers the use of ab10901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Can be blocked with Human FOXP3 peptide (ab16809).
IHC-P Use a concentration of 0.3 - 1 µg/ml.
ICC/IF Use a concentration of 2 - 4 µg/ml.
ChIP Use at an assay dependent concentration.


  • FunctionProbable transcription factor. Plays a critical role in the control of immune response.
  • Involvement in diseaseDefects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • Sequence similaritiesContains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIID antibody
    • DIETER antibody
    • Forkhead box P3 antibody
    • Forkhead box protein P3 antibody
    • FOXP3 antibody
    • FOXP3_HUMAN antibody
    • FOXP3delta7 antibody
    • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
    • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
    • IPEX antibody
    • JM2 antibody
    • MGC141961 antibody
    • MGC141963 antibody
    • OTTHUMP00000025832 antibody
    • OTTHUMP00000025833 antibody
    • OTTHUMP00000226737 antibody
    • PIDX antibody
    • Scurfin antibody
    • XPID antibody
    see all

Anti-FOXP3 antibody images

  • All lanes : Anti-FOXP3 antibody (ab10901) at 0.4 µg/ml

    Lane 1 : 20µg HEK 293 lysate
    Lane 2 : 20µg HEK 293 lysate over expressing human FOXP3
    Lane 3 : 20µg HEK 293 lysate over expressing mouse FOXP3
    Lane 4 : 20µg HEK 293 lysate with Human FOXP3 peptide (ab16809) at 1 µg/ml
    Lane 5 : 20µg HEK 293 lysate over expressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml
    Lane 6 : 20µg HEK 293 lysate over expressing mouse FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml

    Lanes 4 - 6 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 0.2 µg/ml

    Performed under reducing conditions.

    Predicted band size : 47 kDa

    Exposure time : 1 minute

    A no primary control lane showed no bands.

References for Anti-FOXP3 antibody (ab10901)

This product has been referenced in:
  • Mehra S  et al. The Mycobacterium tuberculosis stress response factor SigH is required for bacterial burden as well as immunopathology in primate lungs. J Infect Dis 205:1203-13 (2012). IHC ; Non human primates . Read more (PubMed: 22402035) »
  • Oh U  et al. Reduced Foxp3 Protein Expression Is Associated with Inflammatory Disease during Human T Lymphotropic Virus Type 1 Infection. J Infect Dis 193:1557-66 (2006). Read more (PubMed: 16652285) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

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Submitted Apr 07 2009