This antibody recognises a single clean band representing FOXP3 on HEK293 cell lysate overexpressing human FOXP3. This band is blocked by the immunising peptide and is not present in the control HEK293 cell lysate.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Can be blocked with Human FOXP3 peptide (ab16809).
Use a concentration of 0.3 - 1 µg/ml.
Use a concentration of 2 - 4 µg/ml.
Use at an assay dependent concentration.
Probable transcription factor. Plays a critical role in the control of immune response.
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
Immune dysregulation polyendocrinopathy enteropathy X linked antibody
Immunodeficiency polyendocrinopathy enteropathy X linked antibody
Western blot - FOXP3 antibody (ab10901)
All lanes : Anti-FOXP3 antibody (ab10901) at 0.4 µg/ml
Lane 1 : 20µg HEK 293 lysate Lane 2 : 20µg HEK 293 lysate over expressing human FOXP3 Lane 3 : 20µg HEK 293 lysate over expressing mouse FOXP3 Lane 4 : 20µg HEK 293 lysate with Human FOXP3 peptide (ab16809) at 1 µg/ml Lane 5 : 20µg HEK 293 lysate over expressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml Lane 6 : 20µg HEK 293 lysate over expressing mouse FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml
Mehra S et al. The Mycobacterium tuberculosis stress response factor SigH is required for bacterial burden as well as immunopathology in primate lungs. J Infect Dis205:1203-13 (2012).
Non human primates
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