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Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse FOXP3.
This antibody specifically recognises mouse FOXP3 and does not recognise human FOXP3.
Our Abpromise guarantee covers the use of ab15590 in the following tested applications.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).Can be blocked with Mouse FOXP3 peptide (ab15736).|
Immunohistochemistical detection of FOXP3 using antibody ab15590 on paraformaldehyde perfusion fixed mouse heart tissue sections. Primary antibody ab15590 diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: Goat anti-rabbit biotin conjugated (1/300). Immunoreactivity visualised by DAB; the antibody produced a puctuated and cytoplasmic staining in cardiac cells.