The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use at an assay dependent concentration.
Probable transcription factor. Plays a critical role in the control of immune response.
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
Immune dysregulation polyendocrinopathy enteropathy X linked antibody
Immunodeficiency polyendocrinopathy enteropathy X linked antibody
Western blot - Anti-FOXP3 antibody (ab4728)
Predicted band size : 47 kDa
Western blot using ab4728 at 1/500.
Lane 1: HEK 293 lysate with ab4728 Lane 2: HEK 293 lysate over expressing human FOXP3 with ab4728 Lane 3: HEK 293 lysate over expressing mouse FOXP3 with ab4728 Lane 4: HEK 293 lysate with secondary antibody control ab6721.
Secondary antibody: Goat polyclonal to Rabbit IgG (HRP) ab6721 1/5000. Exposure time: 30 sec. Expected molecular weight: 47kD
IHC image of FOXP3 staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4728, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Hvarness T et al. Phenotypic characterisation of immune cell infiltrates in testicular germ cell neoplasia. J Reprod Immunol100:135-45 (2013).
Read more (PubMed: 24290033) »