Anti-FOXP3 antibody (ab75763)
Key features and details
- Rabbit polyclonal to FOXP3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-FOXP3 antibody
See all FOXP3 primary antibodies -
Description
Rabbit polyclonal to FOXP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Cat, Human, Pig, Monkey, Cynomolgus monkey, Rhesus monkey, Deer -
Immunogen
Synthetic peptide corresponding to Mouse FOXP3 aa 200-300. The peptide was post synthetically modified to achieve desired antigenicity before coupling to a carrier protein.
Database link: Q99JB6 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 52-55, 25-29 kDa (predicted molecular weight: 47 kDa).
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IHC-P | (1) |
1/100.
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Notes |
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WB
1/500. Detects a band of approximately 52-55, 25-29 kDa (predicted molecular weight: 47 kDa). |
IHC-P
1/100. |
Target
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Function
Probable transcription factor. Plays a critical role in the control of immune response. -
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 506053 Cow
- Entrez Gene: 102120882 Cynomolgus monkey
- Entrez Gene: 50943 Human
- Entrez Gene: 20371 Mouse
- Entrez Gene: 444998 Pig
- Entrez Gene: 317382 Rat
- Omim: 300292 Human
- SwissProt: Q6U8D7 Cynomolgus monkey
see all -
Alternative names
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
Images
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Immunofluorescence of tumors from mice treated with MN-siPDL1 and gemcitabine. There was evidence of an increase in cell-mediated cytotoxicity, as evidenced by a decrease in the infiltration by immunosuppressive Foxp3 + regulatory T (Treg) cells detected using ab75763.
This image has been cropped from the origanal figure.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Mouse kidney tissue staining FOXP3 using ab75763 at 1/100. DAB staining (brown) Hematoxylin QS counterstain (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Mouse kidney tissue staining FOXP3 using ab75763 at 1/100. DAB staining (brown) Hematoxylin QS counterstain (blue).
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All lanes : Anti-FOXP3 antibody (ab75763) at 1/500 dilution
Lane 1 : rat kidney lysate
Lane 2 : rat cerebral cortex lysate
Predicted band size: 47 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat kidney tissue staining FOXP3 using ab75763 at 1/100. DAB staining (brown) Hematoxylin QS counterstain (blue).
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab75763 has been referenced in 7 publications.
- Fang Z et al. CS12192, a Novel JAK3/JAK1/TBK1 Inhibitor, Synergistically Enhances the Anti-Inflammation Effect of Methotrexate in a Rat Model of Rheumatoid Arthritis. Int J Mol Sci 23:N/A (2022). PubMed: 36362183
- Zhou J et al. Taurine promotes the production of CD4+CD25+FOXP3+ Treg cells through regulating IL-35/STAT1 pathway in a mouse allergic rhinitis model. Allergy Asthma Clin Immunol 17:59 (2021). PubMed: 34147127
- Zhang Y et al. Hydrogel-load exosomes derived from dendritic cells improve cardiac function via Treg cells and the polarization of macrophages following myocardial infarction. J Nanobiotechnology 19:271 (2021). PubMed: 34496871
- Li W et al. Lymphatic drainage from bronchus-associated lymphoid tissue in tolerant lung allografts promotes peripheral tolerance. J Clin Invest 130:6718-6727 (2020). PubMed: 33196461
- Yoo B et al. RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy. Sci Rep 9:4712 (2019). IHC-P ; Mouse . PubMed: 30886310
- Ma Y et al. Hydroxyl safflower yellow A regulates the tumor immune microenvironment to produce an anticancer effect in a mouse model of hepatocellular carcinoma. Oncol Lett 17:3503-3510 (2019). Flow Cyt . PubMed: 30867790
- Lockett NN et al. Expression of regulatory T cell (Treg) activation markers in endometrial tissues from early and late pregnancy in the feline immunodeficiency virus (FIV)-infected cat. Placenta 31:796-802 (2010). IHC-FoFr ; Cat . PubMed: 20674972