Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXP3
  • Tested applications
    Suitable for: IHC-FoFr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Cat, Human, Monkey
    Predicted to work with: Pig, Cynomolgus monkey, Rhesus monkey, Deer
  • Immunogen

    Synthetic peptide:

    AQCLLQREVVQSLEQQLELEKE

    , corresponding to amino acids 229-250 of Mouse FOXP3. The peptide was post synthetically modified to achieve desired antigenicity before coupling to a carrier protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab75763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20674972
WB 1/500. Detects a band of approximately 52-55, 25-29 kDa (predicted molecular weight: 47 kDa).
IP 1/200. inc. pull down assays (2-5 µl/250 µg protein extracts)

Target

  • Function
    Probable transcription factor. Plays a critical role in the control of immune response.
  • Involvement in disease
    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIID antibody
    • DIETER antibody
    • Forkhead box P3 antibody
    • Forkhead box protein P3 antibody
    • FOXP3 antibody
    • FOXP3_HUMAN antibody
    • FOXP3delta7 antibody
    • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
    • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
    • IPEX antibody
    • JM2 antibody
    • MGC141961 antibody
    • MGC141963 antibody
    • OTTHUMP00000025832 antibody
    • OTTHUMP00000025833 antibody
    • OTTHUMP00000226737 antibody
    • PIDX antibody
    • Scurfin antibody
    • XPID antibody
    see all

References

This product has been referenced in:
  • Lockett NN  et al. Expression of regulatory T cell (Treg) activation markers in endometrial tissues from early and late pregnancy in the feline immunodeficiency virus (FIV)-infected cat. Placenta 31:796-802 (2010). IHC-FoFr ; Cat . Read more (PubMed: 20674972) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Intestinal tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Specification
Intestinal tissue
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
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Submitted Aug 04 2017

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