The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Probable transcription factor. Plays a critical role in the control of immune response.
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
Immune dysregulation polyendocrinopathy enteropathy X linked antibody
Immunodeficiency polyendocrinopathy enteropathy X linked antibody
Western blot - Anti-FOXP3 [EPR15038-69] antibody - C-terminal (ab191416)
All lanes : Anti-FOXP3 antibody [EPR15038-69] (ab191416) at 5 µg/ml
Lane 1 : HEK293T cell lysate Lane 2 : HEK293T cell lysate overexpressing Human FOXP3
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab10563. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Immunohistochemical analysis of paraffin-embedded Human Hodgkin lymphoma tissue labeling FOXP3 with ab191416 at 1/250 dilution (0.9 μg/ml) followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.