Overview

  • Product nameAnti-FOXP3 antibody [mAbcam 450]
    See all FOXP3 primary antibodies
  • Description
    Mouse monoclonal [mAbcam 450] to FOXP3
  • SpecificityDetects a single clean band on human FOXP3 transfected 293 cells. When used in immunocytochemistry on acetone fixed FOXP protein transfected COS cells the antibody only detects FOXP3 and not FOXP1, FOXP2 or FOXP4.
  • Tested applicationsSuitable for: IP, IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human FOXP3 aa 400 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9BZS1
    (Peptide available as ab16809)

Properties

Applications

Our Abpromise guarantee covers the use of ab450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. Used at 2 µg/ml on human FOXP3 transfected COS cells (see Abreview for further information).
IHC-P 1/50.
This clone has been tested against FOXP3 transfectants of COS-1 cells fixed with acetone and also works against formalin-fixed paraffin-embedded FOXP3 transfectants heat retrieved with pH9 Tris-EDTA buffer. It did not show positive staining with cryostat or paraffin-embedded human tonsil.
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human FOXP3 peptide (ab16809).

Target

  • FunctionProbable transcription factor. Plays a critical role in the control of immune response.
  • Involvement in diseaseDefects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • Sequence similaritiesContains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIID antibody
    • DIETER antibody
    • Forkhead box P3 antibody
    • Forkhead box protein P3 antibody
    • FOXP3 antibody
    • FOXP3_HUMAN antibody
    • FOXP3delta7 antibody
    • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
    • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
    • IPEX antibody
    • JM2 antibody
    • MGC141961 antibody
    • MGC141963 antibody
    • OTTHUMP00000025832 antibody
    • OTTHUMP00000025833 antibody
    • OTTHUMP00000226737 antibody
    • PIDX antibody
    • Scurfin antibody
    • XPID antibody
    see all

Anti-FOXP3 antibody [mAbcam 450] images

  • All lanes : Anti-FOXP3 antibody [mAbcam 450] (ab450) at 1 µg/ml

    Lane 1 : HEK293 cells overexpressing human FOXP3
    Lane 2 : HEK293 cells overexpressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 45 seconds
  • Clone mAbcam 450 used in immunocytochemistry on transfected COS-1 cytospins fixed with acetone for 10 mins.

    1: FOXP1 transfectant
    2: FOXP2 transfectant
    3: FOXP3 transfectant
    4: FOXP4 transfectant

    Staining can be seen on the FOXP3 transfectant, but no staining is seen with the other FOXP protein transfectants, confirming the specificity of this antibody in immunocytochemistry.

    These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant).

  • Clone mAbcam 450 used in immunocytochemistry on paraffin embedded transfected COS-1 cytospins.

    1: FOXP1 transfectant
    2: FOXP2 transfectant
    3: FOXP3 transfectant
    4: FOXP4 transfectant

    Strong staining can be seen on the FOXP3 transfectant, with very weak staining on the FOXP1 transfectant and no staining is seen with the other FOXP protein transfectants. This shows that the antibody is largely specific for FOXP3 when used with paraffin embedded cells.

    These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant).

References for Anti-FOXP3 antibody [mAbcam 450] (ab450)

This product has been referenced in:
  • Han S  et al. Tumour-infiltrating CD4(+) and CD8(+) lymphocytes as predictors of clinical outcome in glioma. Br J Cancer 110:2560-8 (2014). Read more (PubMed: 24691423) »
  • Wang L  et al. Somatic single hits inactivate the X-linked tumor suppressor FOXP3 in the prostate. Cancer Cell 16:336-46 (2009). WB ; Human . Read more (PubMed: 19800578) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Human FOXP3 transfected COS cells)
Specification Human FOXP3 transfected COS cells
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Jan 26 2006

Thank you for your enquiry. ab450, ab20034, ab22510, ab22511 have all been tested and shown to work on formalin fixed tissue. However, may I recommend the monoclonal, ab22510 FOXP3 antibody [mAbcam 22510]. This has been used in the following public...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"