The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-F: 1/100 - 1/300.
WB: Use at a concentration of 1 µg/ml (Human FOXP4 Partial Recombinant Protein). Used at a dilution of 1/1000 for 12 hrs incubation on human cells (see Abreview for further details).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Transcriptional repressor that represses lung-specific expression.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-FOXP4 antibody (ab17726)This image is courtesy of Shunyou Wang, Ph.D.
ab17726 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) stomach. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/100 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
Western blot - FOXP4 antibody (ab17726)This image is courtesy of an anonymous Abreview
All lanes : Anti-FOXP4 antibody (ab17726) at 1/1000 dilution
Lane 1 : 50ug Lung epithelial cell line, non-transfected. Lane 2 : 50ug Lung epithelial cell line, transfected with FOXP4.
Secondary All lanes : HRP cpnjugated goat anti-rabbit
Spiteri E et al. Identification of the transcriptional targets of FOXP2, a gene linked to speech and language, in developing human brain. Am J Hum Genet81:1144-57 (2007).
Read more (PubMed: 17999357) »