Overview

  • Product nameAnti-FOXP4 antibody
    See all FOXP4 primary antibodies
  • Description
    Rabbit polyclonal to FOXP4
  • Tested applicationsSuitable for: IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human FOXP4.

    (Peptide available as ab20334.)

  • Positive control
    • Human FOXP4 Partial Recombinant Protein (tagged) Mouse stomach tissue lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
WB
  • Application notesIHC-F: 1/100 - 1/300.
    WB: Use at a concentration of 1 µg/ml (Human FOXP4 Partial Recombinant Protein). Used at a dilution of 1/1000 for 12 hrs incubation on human cells (see Abreview for further details).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional repressor that represses lung-specific expression.
    • Sequence similaritiesContains 1 C2H2-type zinc finger.
      Contains 1 fork-head DNA-binding domain.
    • DomainThe leucine-zipper is required for dimerization and transcriptional repression.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1200010K03Rik antibody
      • 2310007G05Rik antibody
      • FKHLA antibody
      • FLJ40908 antibody
      • FLJ44184 antibody
      • Fork head related protein like A antibody
      • Fork head-related protein-like A antibody
      • Forkhead box P4 antibody
      • Forkhead box protein P4 antibody
      • FOX P4 antibody
      • FOXP 4 antibody
      • foxp4 antibody
      • FOXP4_HUMAN antibody
      • hFKHLA antibody
      • mFKHLA antibody
      • OTTHUMP00000016374 antibody
      • OTTHUMP00000039784 antibody
      • OTTHUMP00000043412 antibody
      • OTTHUMP00000043536 antibody
      • Winged helix repressor FOX P4 antibody
      • Winged helix repressor FOXP 4 antibody
      • Winged helix repressor FOXP4 antibody
      see all

    Anti-FOXP4 antibody images

    • ab17726 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) stomach. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/100 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
    • All lanes : Anti-FOXP4 antibody (ab17726) at 1/1000 dilution

      Lane 1 : 50ug Lung epithelial cell line, non-transfected.
      Lane 2 : 50ug Lung epithelial cell line, transfected with FOXP4.

      Secondary
      HRP cpnjugated goat anti-rabbit

      Performed under reducing conditions.

      Predicted band size : 11 kDa


      Exposure time : 1 second

      This image is courtesy of an anonymous Abreview

      See Abreview

    • Anti-FOXP4 antibody (ab17726) at 1 µg/ml + Human FOXP4 Partial Recombinant Protein (Tagged) at 0.1 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 11 kDa
      Observed band size : 11 kDa

    References for Anti-FOXP4 antibody (ab17726)

    ab17726 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (lung epithelial cell line)
    Loading amount 50 µg
    Specification lung epithelial cell line
    Treatment transfected
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Jul 06 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"