Overview

  • Product nameAnti-FOXP4 antibody
    See all FOXP4 primary antibodies
  • Description
    Mouse monoclonal to FOXP4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment: LNSPGMLNPG SASSLLPLSH DDVGAPVEPL PSNGSSSPPR LSPPQYSHQV QVKEEPAEAE EDRQPGPPLG APNPSASGPP EDRDLEEELP GEEL, corresponding to amino acids 586-680 of Human FOXP4 (NP_001012426) with tag.

  • Positive control
    • FOXP4 transfected 293T cell lysate; Recombinant tagged human FOXP4 fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 74 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionTranscriptional repressor that represses lung-specific expression.
  • Sequence similaritiesContains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • DomainThe leucine-zipper is required for dimerization and transcriptional repression.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1200010K03Rik antibody
    • 2310007G05Rik antibody
    • FKHLA antibody
    • FLJ40908 antibody
    • FLJ44184 antibody
    • Fork head related protein like A antibody
    • Fork head-related protein-like A antibody
    • Forkhead box P4 antibody
    • Forkhead box protein P4 antibody
    • FOX P4 antibody
    • FOXP 4 antibody
    • foxp4 antibody
    • FOXP4_HUMAN antibody
    • hFKHLA antibody
    • mFKHLA antibody
    • OTTHUMP00000016374 antibody
    • OTTHUMP00000039784 antibody
    • OTTHUMP00000043412 antibody
    • OTTHUMP00000043536 antibody
    • Winged helix repressor FOX P4 antibody
    • Winged helix repressor FOXP 4 antibody
    • Winged helix repressor FOXP4 antibody
    see all

Anti-FOXP4 antibody images

  • Anti-FOXP4 antibody (ab77141) at 1 µg/ml + Recombinant tagged human FOXP4 fragment at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 74 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-FOXP4 antibody (ab77141) at 1 µg/ml

    Lane 1 : FOXP4 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 74 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)

References for Anti-FOXP4 antibody (ab77141)

ab77141 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"