A region within synthetic peptide: GDDSLGSDGD CAANSPAAGG GARDPPGDGE QSAGGGPGAE EAIPAAAAAA, corresponding to N terminal amino acids 36-85 of Human FOXQ1
Our Abpromise guarantee covers the use of ab51340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA | Use at an assay dependent concentration. | |
ICC/IF | Use a concentration of 5 µg/ml. | |
WB | Use a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
IHC-P | Use a concentration of 4 - 8 µg/ml. |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FOXQ1 with ab51340 at 4-8µg/ml. Arrows indicate postively labelled epithelial cells of renal tubule. Magnification: 400X.
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