Anti-FOXQ1 antibody (ab51340)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-FOXQ1 antibody
See all FOXQ1 primary antibodies -
DescriptionRabbit polyclonal to FOXQ1
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
A region within synthetic peptide: GDDSLGSDGD CAANSPAAGG GARDPPGDGE QSAGGGPGAE EAIPAAAAAA, corresponding to N terminal amino acids 36-85 of Human FOXQ1
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Positive control
- Jurkat cell lysate and human kidney tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab51340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 4 - 8 µg/ml. |
Target
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Tissue specificityExpressed predominantly in the stomach, trachea, bladder and salivary gland.
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Sequence similaritiesContains 1 fork-head DNA-binding domain.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 94234 Human
- Omim: 612788 Human
- SwissProt: Q9C009 Human
- Unigene: 591352 Human
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Alternative names
- Forkhead box protein Q1 antibody
- Forkhead box Q1 antibody
- FOX Q1 antibody
see all
Images
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Western blot - Anti-FOXQ1 antibody (ab51340)Anti-FOXQ1 antibody (ab51340) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with skim milk/ PBS at 5 %
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 50 kDa (why is the actual band size different from the predicted?)
Gel concentration 12% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXQ1 antibody (ab51340)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FOXQ1 with ab51340 at 4-8µg/ml. Arrows indicate postively labelled epithelial cells of renal tubule. Magnification: 400X.
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Immunocytochemistry/ Immunofluorescence - Anti-FOXQ1 antibody (ab51340)ICC/IF image of ab51340 stained cells. The cells were and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Qin A et al. MicroRNA-1271 inhibits cellular proliferation of hepatocellular carcinoma. Oncol Lett 14:6783-6788 (2017). Read more (PubMed: 29181102) »
- Yonemori K et al. The microRNA expression signature of pancreatic ductal adenocarcinoma by RNA sequencing: anti-tumour functions of themicroRNA-216cluster. Oncotarget 8:70097-70115 (2017). Read more (PubMed: 29050264) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"