Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXR2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: PFPQPPQKDE GSNCSEDKVV ESLPSSSSEQ SPLQKQGIHS PSDFELTEEE, corresponding to internal sequence amino acids 109-158 of Human FOXR2

  • Positive control
    • Jurkat cell lysate; human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab55961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FOXR2 with ab55961 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

  • Lane 1 : marker
    Lane 2 : Anti-FOXR2 antibody (ab55961) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP-labelled anti-rabbit IgG at 1/50000 dilution


    Predicted band size : 36 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%

References

ab55961 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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