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Synthetic peptide derived from residues 1 - 50 of Mouse Fragilis.
(Peptide available as ab15737.)
Our Abpromise guarantee covers the use of ab15592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. PubMed: 21615296|
|IHC-G||Use at an assay dependent concentration. PubMed: 22446628|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-Fr||Use a concentration of 10 µg/ml.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).Can be blocked with Fragilis peptide (ab15737).|
IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).
ab15592 (anti-Fragilis) = red
Oct4 = green
DAPI (DNA) = blue
The dilution of ab15592 used was 10 ug/ml.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"