Overview

  • Product nameAnti-Fragilis antibodySee all Fragilis primary antibodies ...
  • Description
    Rabbit polyclonal to Fragilis
  • Tested applicationsIP, IHC-P, IHC-G, ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from residues 1 - 50 of Mouse Fragilis.

    (Peptide available as ab15737.)

  • Positive control
    • WB: Mouse Embryonic Stem Cell lysate, Mouse Embryonic Germ Cell lysate IHC-Fr: male genital ridges of E12.5 mouse embryos

Properties

Applications

Our Abpromise guarantee covers the use of ab15592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 21615296
IHC-G Use at an assay dependent concentration. PubMed: 22446628
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).Can be blocked with Fragilis peptide (ab15737).

Target

  • FunctionIFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication.
  • Sequence similaritiesBelongs to the CD225 family.
  • Post-translational
    modifications
    Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 8U antibody
    • Fragilis antibody
    • Ifitm 3 antibody
    • Ifitm3 antibody
    • IFM3_HUMAN antibody
    • Interferon induced transmembrane protein 3 (1 8U) antibody
    • Interferon Induced Transmembrane Protein 3 antibody
    • Interferon inducible antibody
    • Interferon inducible protein 1 8U antibody
    • Interferon Inducible Protein 15 antibody
    • Interferon Inducible Protein Homolog antibody
    • Interferon-induced transmembrane protein 3 antibody
    • Interferon-inducible protein 1-8U antibody
    • IP15 antibody
    see all

Anti-Fragilis antibody images

  • All lanes : Anti-Fragilis antibody (ab15592) at 1 µg/ml

    Lane 1 : Mouse Embryonic Stem Cell lysate
    Lane 2 : Mouse Embryonic Germ Cell lysate
    Lane 3 : Mouse Embryonic Stem Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml
    Lane 4 : Mouse Embryonic Germ Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 15 kDa
    Observed band size : 13 kDa (why is the actual band size different from the predicted?)
  • IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).

    ab15592 (anti-Fragilis) = red
    Oct4 = green
    DAPI (DNA) = blue

    The dilution of ab15592 used was 10 ug/ml.

  • Anti-Fragilis antibody, ab15592, recognised a small proportion of cells in a culture of human embryonic stem cells differentiated into a heterogenous mix of cells. Based upon the sequence of the immunogen used to generate this antibody the protein it recognizes is likely to be IFITM2 and/or IFITM3.
  • Fragilis - Primordial Germ Cell Marker was immunoprecipitated using 0.5mg E14Tg2a whole cell extract, 5µg of Rabbit polyclonal to Fragilis and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, E14Tg2a whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15592.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 13kDa: Fragilis - Primordial Germ Cell Marker.

References for Anti-Fragilis antibody (ab15592)

This product has been referenced in:
  • Zhou L  et al. Production of fat-1 transgenic rats using a post-natal female germline stem cell line. Mol Hum Reprod 20:271-81 (2014). Rat . Read more (PubMed: 24258451) »
  • Wakim LM  et al. Enhanced survival of lung tissue-resident memory CD8? T cells during infection with influenza virus due to selective expression of IFITM3. Nat Immunol 14:238-45 (2013). WB . Read more (PubMed: 23354485) »

See all 10 Publications for this product

Product Wall

He llevado a cabo la alineación de secuencias de la secuencia que me has enviado con la correspondiente al inmunógeno de ab74699 y resulta un porcentaje del 66%:

Sequence type explicitly set to Protein

Sequence format ...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Apr 13 2012

Thank you for your enquiry. I have looked at the amino acid sequence used to generate the antibody and can confirm it is at the N terminus of the protein, however the SwissProt reference we currently have (Q9D8L6) does not specify which part of the pro...

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