Overview

  • Product name
  • Description
    Rabbit polyclonal to Fragilis
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide derived from an internal sequence of human Fragilis.

  • Positive control
    • HeLa and HepG2 cell extracts. MCF-7 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab74699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 15 kDa.
ICC/IF 1/500 - 1/1000.
ELISA 1/40000.

Target

  • Function
    IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication.
  • Sequence similarities
    Belongs to the CD225 family.
  • Post-translational
    modifications
    Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 8U antibody
    • Fragilis antibody
    • IFITM3 antibody
    • IFM3_HUMAN antibody
    • Interferon induced transmembrane protein 3 (1 8U) antibody
    • Interferon Induced Transmembrane Protein 3 antibody
    • Interferon inducible antibody
    • Interferon inducible protein 1 8U antibody
    • Interferon Inducible Protein 15 antibody
    • Interferon Inducible Protein Homolog antibody
    • Interferon-induced transmembrane protein 3 antibody
    • Interferon-inducible protein 1-8U antibody
    • IP15 antibody
    see all

Images

  • All lanes : Anti-Fragilis antibody (ab74699) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells (5-30µg)
    Lane 2 : Extracts from HeLa cells (5-30µg)
    Lane 3 : Extracts from HeLa cells (5-30µg), plus immunising peptide (5-10µg)


    Predicted band size : 15 kDa
    Observed band size : <19 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent staining of MCF-7 cells, using ab74699 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).

References

This product has been referenced in:
  • White YA  et al. Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women. Nat Med 18:413-21 (2012). Read more (PubMed: 22366948) »
  • Bhartiya D  et al. Very Small Embryonic- like Stem Cells with Maximum Regenerative Potential get Discarded during Cord Blood Banking and Bone Marrow Processing for Autologous Stem Cell Therapy. Stem Cells Dev : (2011). ICC/IF ; Human . Read more (PubMed: 21780911) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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