Anti-Fragilis antibody [EPR5242] (ab109429)


  • Product name
    Anti-Fragilis antibody [EPR5242]
    See all Fragilis primary antibodies
  • Description
    Rabbit monoclonal [EPR5242] to Fragilis
  • Specificity
    Predicted cross-reactivity with IFITM2 (93% homology) but this has not been tested.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • HeLa, 293T, and Human placenta lysates; Human kidney tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Alternative versions available:

    Anti-Fragilis antibody (Alexa Fluor® 488) [EPR5242] (ab198559)
    Anti-Fragilis antibody (Alexa Fluor® 647) [EPR5242] (ab198573)

    Anti-Fragilis antibody (HRP) [EPR5242] (ab199021)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab109429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication.
    • Sequence similarities
      Belongs to the CD225 family.
    • Post-translational
      Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 8U antibody
      • Fragilis antibody
      • IFITM3 antibody
      • IFM3_HUMAN antibody
      • Interferon induced transmembrane protein 3 (1 8U) antibody
      • Interferon Induced Transmembrane Protein 3 antibody
      • Interferon inducible antibody
      • Interferon inducible protein 1 8U antibody
      • Interferon Inducible Protein 15 antibody
      • Interferon Inducible Protein Homolog antibody
      • Interferon-induced transmembrane protein 3 antibody
      • Interferon-inducible protein 1-8U antibody
      • IP15 antibody
      see all

    Anti-Fragilis antibody [EPR5242] images

    • All lanes : Anti-Fragilis antibody [EPR5242] (ab109429) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 15 kDa
    • Immunohistochemical analysis of Fragilis in paraffin-embedded Human kidney tissue using ab109429 at 1/250 dilution.
    • Anti-Fragilis antibody [EPR5242] (ab109429) at 1/1000 dilution + Recombinant Human Fragilis protein (ab92921) at 0.01 µg

      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds
    • ICC/IF image of ab109429 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109429 at 1/50 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Fragilis antibody [EPR5242] (ab109429)

    ab109429 has not yet been referenced specifically in any publications.

    Product Wall

    Immunocytochemistry/ Immunofluorescence
    Cow Cell (Blastocyst Day7)
    Yes - 0,2% Triton X
    Blastocyst Day7
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Irene Röttinger

    Verified customer

    Submitted Nov 30 2016

    Thank you for contacting Abcam.

    The immunogen for cat.# ab109429 has a 93% homology with IFITM2. However, we have not tested any potential cross-reactivity with IFITM2. I would predict cross-reactivity, but it is not guaranteed wit...

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