Anti-Francisella tularensis LPS antibody [FB11] (ab2033)

Overview

  • Product nameAnti-Francisella tularensis LPS antibody [FB11]
    See all Francisella tularensis LPS primary antibodies
  • Description
    Mouse monoclonal [FB11] to Francisella tularensis LPS
  • Specificityab2033 recognises LPS of virulent and vaccine strains of Fr. tularensis. There is no cross-reactivity  with Fr. Novicida, Br. abortus, Br. suis, Br. melitensis, Br. ovis, Y. pestis, Y. enterocolitica, Y. pseudotuberculosis, E.Coli, V. cholerae, B.neotomae, S.typhimurium.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Spleen cells of Balb/c mice were immunised with purified F.tularensis strain 15 LPS.

  • EpitopeThe binding site for ab2033 is located on the O-antigen polysaccharide chain which consists of tetrasaccharide fragments and has the following structure: -4)-D-GalpNAcAN-(1-4)--D-GalpNAcAN-(1-3)--D-QuipNac-(1-2)--Quip4NFm-(1. Tetrasaccharide D-GalpNAcAN-(1-4)--D-GalpNAcAN-(1-3)--D-QuipNAc-(1-2)--D-Quip4NFm and trisaccharide D-GalpNAcAN-(1-3)--D-QuipNAc-(1-2)--D-Quip4NFm compete in ELISA for binding MoAb FB11 with LPS of Fr. tularensis.
  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab2033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 21846829
ELISA Use at an assay dependent dilution. The detection limit for the immunoassay is 0.125 ng/ml.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceFrancisella tularensis is a small, nonmotile, aerobic, gram-negative coccobacillus. It has a thin lipopolysaccharide-containing envelope and is a hardy non–spore-forming organism that survives for weeks at low temperatures in water, moist soil, hay, straw, and decaying animal carcasses. Lipopolysaccaride (LPS) is a main species-specific antigen of Francisella tularensis. According to the data available LPS of tularemia microbe differs from LPS of other gram-negative bacteria. LPS of tularemia microbe hasn’t yet revealed the properties of classical endotoxin, which may be connected with non-typical structure of lipid A – a toxic component of LPS.
  • Alternative names
    • Lipopolysaccharide antibody
    • Lipopolysaccharide protein antibody

Anti-Francisella tularensis LPS antibody [FB11] images

  • Image from Kim TH et al, J Biol Chem. 2010 Sep 3;285(36):27839-49. Epub 2010 Jul 6, Fig 2.

References for Anti-Francisella tularensis LPS antibody [FB11] (ab2033)

This product has been referenced in:
  • Wu X  et al. FmvB: A Francisella tularensis Magnesium-Responsive Outer Membrane Protein that Plays a Role in Virulence. PLoS One 11:e0160977 (2016). WB . Read more (PubMed: 27513341) »
  • Gillette DD  et al. Virulent Type A Francisella tularensis actively suppresses cytokine responses in human monocytes. Front Cell Infect Microbiol 4:45 (2014). ICC/IF . Read more (PubMed: 24783062) »

See all 14 Publications for this product

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I can confirm that ab2033 and ab60295 are different antibodies raised against different immunogens . Please note that ab2033 is IgG2aisotype and ab60295 is IgG1 isotype, and this will need to be taken into conside...

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We would not recommend ab97234 as a secondary for ab2033 because they do not match in isotype. The secondary ab97234 targets Mouse IgA, but the primary ab2033 is IgG2a.


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Thank you for your enquiry, I'm delighted to hear you are satisfied with ab2033. Would you consider submitting an Abreview? In return we will offer you 50 Abpoints which can be redeemed on a number of rewards (a further 100 Abpoints will be offered fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"