Overview

  • Product name
    Anti-FRAT2 antibody
  • Description
    Goat polyclonal to FRAT2
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: GPDRIALQPSGSLL, corresponding to C terminal amino acids 220-233 of Human FRAT2.

  • Positive control
    • Human Heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab2470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human FRAT2 peptide (ab22910).

Approx 30-35kDa band seen in Human Heart lysate.

Target

  • Database links
  • Alternative names
    • Frequently rearranged in advanced T-cell lymphomas 2 antibody
    • GSK-3 binding protein FRAT2 antibody

Images

  • ab2470 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

    ab2470 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References

ab2470 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1. We incubate the membrane with the primary antibody for 1 hr, then apply 2 washes and 3 washes after the secondary antibody. We would suggest adding 0.05% Tween20 to the blocking solution. 2.We use ECLPlus (Amersham) rather than ECL. ECLPlus is kn...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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