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Recombinant full length mature protein, corresponding to amino acids 56-210 of Human Frataxin.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Although this antibody does work in Western blot, we have found that ab113691 typically gives a more robust signal. Therefore, if you plan on using this antibody in WB only, we recommend ab113691 as an alternative.
Our Abpromise guarantee covers the use of ab110328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 3 - 5 µg/ml. Predicted molecular weight: 23 kDa. (A chemiluminescent detection system is highly recommended)|
|ICC/IF||Use a concentration of 0.5 µg/ml.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 10 µg/ml.|
IHC image of Frataxin staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110328, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.