Overview

  • Product name
    Free Fatty Acid Assay Kit - Quantification
    See all Fatty acid kits
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Quantitative
  • Sensitivity
    > 2 µM
  • Assay time
    0h 40m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Species independent
  • Product overview

    Abcam's Free Fatty Acid Quantification Assay Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids (FA) in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In this assay, FA are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ= 570 nm) or fluorometric (at Ex/Em= 535/587 nm)
    Visit our FAQs page for tips and troubleshooting.


    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • Notes

    This assay detects formation of C-8 (octanoate) and longer fatty acids.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab65341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Plasma FFA levels was measured using ab65341 in male and female wild-type (WT) or AT2KO mice either on normal diet (ND) or high fat diet (HFD).

  • Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

  • Free Fatty Acid measured in biologicals showing concentration (µM)

  • Flourometric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

Protocols

References

This product has been referenced in:
  • Okumura T  et al. Extra-pancreatic invasion induces lipolytic and fibrotic changes in the adipose microenvironment, with released fatty acids enhancing the invasiveness of pancreatic cancer cells. Oncotarget 8:18280-18295 (2017). Mouse . Read more (PubMed: 28407685) »
  • Domingo-Espín J  et al. Dual Actions of Apolipoprotein A-I on Glucose-Stimulated Insulin Secretion and Insulin-Independent Peripheral Tissue Glucose Uptake Lead to Increased Heart and Skeletal Muscle Glucose Disposal. Diabetes 65:1838-48 (2016). Read more (PubMed: 27207515) »

See all 25 Publications for this product

Customer reviews and Q&As


Although we would suggest to use fresh tissue preferably, tissue that is snap-frozen in liquid nitrogen can be used too. The tissue can be ground and then lipid extraction can be done with choloroform-triton.

Abreviews
Plasma from yellow-bellied marmots (Marmota flaviventris) was processed according the protocol. A serial dilution of the plasma (shown) was compared to the standard curved (also shown) to determine the optimal dilution factor for subsequent assays.
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Abcam user community

Verified customer

Submitted Jul 20 2016

No, the kit does not detect triglycerides or other molecules that have a fatty acid components. The triglycerides need an enzyme to convert them to free fatty acids and this is not present in any of the kit components.

The reason heparinized plasma is not recommended for the free fatty acid assay is because heparin releases lipolytic enzymes that lead to an overestimation of FFA.

You can test for endogoenous Acyl-CoAs in your samples by including sample wells without added ACS Reagent. In these wells, the long-chain free fatty acids will not be converted to coenzyme A, so if there are any interfering compounds including endogen...

Read More

We would recommend fresh serum samples for best results. If fresh samples is not an option storage at -80 °C is less likely to degrade the sample quality.

For this product ab65341, blood needs to be collected using an anticoagulant such as...

Read More

Blood needs to be collected using an anticoagulant such as EDTA, sodium citrate, sodium fluoride, or ammonium oxalate. Heparinized plasma is not the best choice as heparin could interfere with the assay.

The details of the assay buffer is considered proprietary information. I can give you a pH range. It is between 7.5-8.2


This kit is good for C-8 or longer fatty acids so it should be suitable for measuring lipoxins.



Our scientists tried this assay with C7 and C6 fatty acids, but found that the final efficiency of detection decreases by ˜50% in that case.

Therefore I can confirm that the minimal length of suitable fatty acid is C8.

1-10 of 34 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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