Overview

  • Product name
    Free Fatty Acid Assay Kit - Quantification
    See all Fatty acid kits
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Quantitative
  • Sensitivity
    > 2 µM
  • Assay time
    0h 40m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Species independent
  • Product overview

    Abcam's Free Fatty Acid Quantification Assay Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids (FA) in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In this assay, FA are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ= 570 nm) or fluorometric (at Ex/Em= 535/587 nm)
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This assay detects formation of C-8 (octanoate) and longer fatty acids.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Relevance
    Fatty acids are carboxylic acids with long hydrocarbon chains. The hydrocarbon chain length may vary from 8-30 carbons. Fatty acids play very important roles in normal metabolism and many disease development. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response.

Applications

Our Abpromise guarantee covers the use of ab65341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Free Fatty Acid Assay Kit - Quantification images

  • Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

  • Free Fatty Acid measured in biologicals showing concentration (µM)

  • Flourometric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

Protocols

References for Free Fatty Acid Assay Kit - Quantification (ab65341)

This product has been referenced in:
  • Okumura T  et al. Extra-pancreatic invasion induces lipolytic and fibrotic changes in the adipose microenvironment, with released fatty acids enhancing the invasiveness of pancreatic cancer cells. Oncotarget 8:18280-18295 (2017). Mouse . Read more (PubMed: 28407685) »
  • Domingo-Espín J  et al. Dual Actions of Apolipoprotein A-I on Glucose-Stimulated Insulin Secretion and Insulin-Independent Peripheral Tissue Glucose Uptake Lead to Increased Heart and Skeletal Muscle Glucose Disposal. Diabetes 65:1838-48 (2016). Read more (PubMed: 27207515) »

See all 25 Publications for this product

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Abreviews
Plasma from yellow-bellied marmots (Marmota flaviventris) was processed according the protocol. A serial dilution of the plasma (shown) was compared to the standard curved (also shown) to determine the optimal dilution factor for subsequent assays.
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Submitted Jul 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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