Overview

  • Product nameFree Fatty Acid Quantification Kit
    See all Fatty acid kits
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 2 µM
  • Assay time
    0h 40m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Species independent
  • Product overview

    Abcam's Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids (FA) in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In this assay, FA are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ= 570 nm) or fluorometric (at Ex/Em= 535/587 nm)
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This assay detects formation of C-8 (octanoate) and longer fatty acids.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevanceFatty acids are carboxylic acids with long hydrocarbon chains. The hydrocarbon chain length may vary from 8-30 carbons. Fatty acids play very important roles in normal metabolism and many disease development. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response.

Applications

Our Abpromise guarantee covers the use of ab65341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Free Fatty Acid Quantification Kit images

  • Free Fatty Acid measured in biologicals showing concentration (µM)

  • Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted

Protocols

References for Free Fatty Acid Quantification Kit (ab65341)

This product has been referenced in:
  • Machado MV  et al. Mouse models of diet-induced nonalcoholic steatohepatitis reproduce the heterogeneity of the human disease. PLoS One 10:e0127991 (2015). Mouse . Read more (PubMed: 26017539) »
  • Lu HJ  et al. Ruscogenin ameliorates experimental nonalcoholic steatohepatitis via suppressing lipogenesis and inflammatory pathway. Biomed Res Int 2014:652680 (2014). Read more (PubMed: 25136608) »

See all 19 Publications for this product

Product Wall

Abreviews
Plasma from yellow-bellied marmots (Marmota flaviventris) was processed according the protocol. A serial dilution of the plasma (shown) was compared to the standard curved (also shown) to determine the optimal dilution factor for subsequent assays.
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Verified customer

Submitted Jul 20 2016

No, the kit does not detect triglycerides or other molecules that have a fatty acid components. The triglycerides need an enzyme to convert them to free fatty acids and this is not present in any of the kit components.

The reason heparinized plasma is not recommended for the free fatty acid assay is because heparin releases lipolytic enzymes that lead to an overestimation of FFA.

You can test for endogoenous Acyl-CoAs in your samples by including sample wells without added ACS Reagent. In these wells, the long-chain free fatty acids will not be converted to coenzyme A, so if there are any interfering compounds including endogen...

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Although we would suggest to use fresh tissue preferably, tissue that is snap-frozen in liquid nitrogen can be used too. The tissue can be ground and then lipid extraction can be done with choloroform-triton.

We would recommend fresh serum samples for best results. If fresh samples is not an option storage at -80 °C is less likely to degrade the sample quality.

For this product ab65341, blood needs to be collected using an anticoagulant such as...

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Blood needs to be collected using an anticoagulant such as EDTA, sodium citrate, sodium fluoride, or ammonium oxalate. Heparinized plasma is not the best choice as heparin could interfere with the assay.

The details of the assay buffer is considered proprietary information. I can give you a pH range. It is between 7.5-8.2


This kit is good for C-8 or longer fatty acids so it should be suitable for measuring lipoxins.



Our scientists tried this assay with C7 and C6 fatty acids, but found that the final efficiency of detection decreases by ˜50% in that case.

Therefore I can confirm that the minimal length of suitable fatty acid is C8.

1-10 of 34 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"