For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Abcam’s Free Glycerol Assay kit II (ab155899) is suitable for measuring free glycerol levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, Glycerol in the presence of Glycerol Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. Free Glycerol Assay Kit II is simple, rapid and high-throughput adaptable. This assay kit can detect less than 20 μM of free Glycerol in various biological samples.
Detection method: colorimetric – based on enzyme activity
Glycerol is a central component of most lipids. It acts as a backbone for triglycerides and phospholipids, which play an important role in metabolism and cell membrane structure. Due to its low toxicity, glycerol is widely used in the pharmaceutical, food and cosmetic industries.
|Glycerol Assay Buffer||WM||1 x 25ml|
|Glycerol Enzyme Mix (lyophilized)||Green||1 vial|
|Glycerol Probe||Red||1 vial|
|Glycerol Standard||Yellow||1 x 0.2ml|
Our Abpromise guarantee covers the use of ab155899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab155899 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"