Overview

  • Product nameFree Estriol Human ELISA Kit
    See all Estriol kits
  • Detection methodColorimetric
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 0.05 ng/ml
  • Range
    0.1 ng/ml - 12 ng/ml
  • Recovery

    101.6 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Free Estriol competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Free Estriol in serum and plasma.

     

    A 96-well plate has been precoated with anti-Estriol IgG. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.

  • Tested applicationsCompetitive ELISAmore details
  • PlatformMicroplate

Properties

  • Alternative names
    • Oestriol

Applications

Our Abpromise guarantee covers the use of ab108671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Free Estriol Human ELISA Kit images

  • Representative Standard Curve using ab108671.

Protocols

References for Free Estriol Human ELISA Kit (ab108671)

ab108671 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. We have a number of antibodies directed against estriol, ab64546, ab20278 and ab44962 are sheep, mouse and rat polyclonals respectively. These have all been tested with radioimmunoassay. Unfortunately we do not have...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"