Free Estriol Human ELISA Kit (ab108671)
- Product nameFree Estriol Human ELISA KitSee all Estriol kits ...
- Detection methodColorimetric
- Tests1 x 96 test
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity= 0.05 ng/ml
- Range0.1 ng/ml - 12 ng/ml
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Free Estriol competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Free Estriol in serum and plasma.
A 96-well plate has been precoated with anti-Estriol IgG. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.
- Tested applicationsCompetitive ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Anti-Estriol IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Estriol Standard 1 – 0.1 ng/mL 1 x 1ml Estriol Standard 2 – 1.0 ng/mL 1 x 1ml Estriol Standard 3 – 4.0 ng/mL 1 x 1ml Estriol Standard 4 – 12.0 ng/mL 1 x 1ml Estriol-HRP conjugate conc 1 x 1ml Incubation buffer 1 x 30ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml
- Research Areas
Our Abpromise guarantee covers the use of ab108671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Free Estriol Human ELISA Kit (ab108671)
ab108671 has not yet been referenced specifically in any publications.