Anti-FREM2 antibody (ab75803)

Overview

  • Product name
  • Description
    Rabbit polyclonal to FREM2
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human FREM2.

    (Peptide available as ab95841.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: EBS E14 TG2A Day 3; EBS E14 TG2A Day 5; EBS E14 TG2A Day 8.

Properties

Applications

Our Abpromise guarantee covers the use of ab75803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 351 kDa (predicted molecular weight: 351 kDa). Also detects a band of approximately 296 kDa, which corresponds to the predicted molecular weight of isoform 2.

Target

  • Function
    Extracellular matrix protein required for maintenance of the integrity of the skin epithelium and for maintenance of renal epithelia. May be required for epidermal adhesion.
  • Involvement in disease
    Defects in FREM2 are a cause of Fraser syndrome (FRASS) [MIM:219000]. Fraser syndrome is a multisystem malformation usually comprising cryptophthalmos, cutaneous syndactyly, ear abnormalities, renal agenesis and congenital heart defects.
  • Sequence similarities
    Belongs to the FRAS1 family.
    Contains 5 Calx-beta domains.
    Contains 12 CSPG (NG2) repeats.
  • Domain
    The Calx-beta domains bind calcium with high affinity and undergo a major conformational shift upon binding.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781I048 antibody
    • ECM3 homolog antibody
    • FRAS1-related extracellular matrix protein 2 antibody
    • FREM 2 antibody
    • Frem2 antibody
    • FREM2_HUMAN antibody
    • KIAA1074 antibody
    see all

Anti-FREM2 antibody images

  • All lanes : Anti-FREM2 antibody (ab75803) at 1 µg/ml

    Lane 1 : EBS E14 TG2A Day 3 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
    Lane 2 : EBS E14 TG2A Day 5 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
    Lane 3 : EBS E14 TG2A Day 8 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 351 kDa
    Observed band size : 351 kDa
    Additional bands at : 100 kDa,296 kDa (possible isoform),45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab75803 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75803, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-FREM2 antibody (ab75803)

ab75803 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK-293 cells)
Specification
HEK-293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 18 2014

Thank you for contacting us. We have not tested ab75803 in Flow Cytometry and do not have any data regarding suitability in this application. You would have to purify the antibody before conjugating it to remove the BSA or the BSA may be conjugated. Read More

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (EpiSC)
Specification
EpiSC
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1 triton-X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Nov 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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