The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionMay have a role in processing of pre-rRNA or in the assembly of rRNA into ribosomal subunits. May be involved in pre-mRNA splicing.
Tissue specificityExpressed in adult muscle, lymphocytes, fetal brain, muscle, and placenta.
Sequence similaritiesBelongs to the FRG1 family.
Cellular localizationNucleus > Cajal body. Nucleus speckle. Nucleus > nucleolus. First expressed in Cajal bodies and nuclear speckles. As speckle expression diminishes, expression is seen in dense structures (dense fibrillar component and the granular component) of the nucleolus.