Overview

  • Product nameAnti-Frizzled 10 antibody
    See all Frizzled 10 primary antibodies
  • Description
    Rabbit polyclonal to Frizzled 10
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis, Chimpanzee, Monkey
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    A PNNGSDEPTR GSGLFPPLFR PQRPHSAQEH PLKDGGPGRG GCDNPGKFHH

    , corresponding to amino acids 150-200 of Human Frizzled 10. (NP_009128).

  • Positive control
    • Human brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab71987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

Target

  • FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificityHighest levels in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. Weak expression detected in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Post-translational
    modifications
    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 350 antibody
    • CD 350 antigen antibody
    • CD350 antibody
    • Frizzled (Drosophila) homolog 10 antibody
    • Frizzled-10 antibody
    • FZ 10 antibody
    • Fz-10 antibody
    • FZ10 antibody
    • FZD 10 antibody
    • FZD10 antibody
    • FZD10_HUMAN antibody
    • FzE 7 antibody
    • FzE7 antibody
    • hFz 10 antibody
    • hFz10 antibody
    see all

Anti-Frizzled 10 antibody images

  • Lane 1 : Pre-immune serum
    Lane 2 : Anti-Frizzled 10 antibody (ab71987) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate


    Predicted band size : 65 kDa
    Observed band size : 65 kDa

References for Anti-Frizzled 10 antibody (ab71987)

ab71987 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Tissue lysate - whole (Eye)
Loading amount 15 µg
Specification Eye
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Nov 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"