Overview

  • Product nameAnti-Frizzled 10 antibody
    See all Frizzled 10 primary antibodies
  • Description
    Rabbit polyclonal to Frizzled 10
  • Tested applicationsSuitable for: IHC-FoFr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Horse, Chicken, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (PIEIPMCKDI GYNMTRMPNL MGHENQREAA IQLHEFAPLV EYGCHGHLRF) of Human Frizzled 10 (NP_009128).

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 23251385
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/62500.

Target

  • FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificityHighest levels in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. Weak expression detected in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • DomainLys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Post-translational
    modifications
    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 350 antibody
    • CD 350 antigen antibody
    • CD350 antibody
    • Frizzled (Drosophila) homolog 10 antibody
    • Frizzled-10 antibody
    • FZ 10 antibody
    • Fz-10 antibody
    • FZ10 antibody
    • FZD 10 antibody
    • FZD10 antibody
    • FZD10_HUMAN antibody
    • FzE 7 antibody
    • FzE7 antibody
    • hFz 10 antibody
    • hFz10 antibody
    see all

Anti-Frizzled 10 antibody images

  • Immunohistochemical image of ab83044 (dilution 1/1000) staining in rat spinal cord PFA fixed frozen tissue section, performed on an Olympus BX61 Motorized Research Microscope. The scale bar represents 50 µm.

  • Anti-Frizzled 10 antibody (ab83044) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration 12%

References for Anti-Frizzled 10 antibody (ab83044)

This product has been referenced in:
  • Gonzalez P  et al. Spatio-temporal expression pattern of frizzled receptors after contusive spinal cord injury in adult rats. PLoS One 7:e50793 (2012). IHC-P ; Rat . Read more (PubMed: 23251385) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"