Overview

  • Product nameAnti-Frizzled 2 antibody
    See all Frizzled 2 primary antibodies
  • Description
    Goat polyclonal to Frizzled 2
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig
  • Immunogen

    Synthetic peptide:

    RYATLEHPFHCPR

    , corresponding to internal sequence amino acids 191-203 of Human Frizzled 2

  • Positive control
    • Human thyroid tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: A549

Properties

Applications

Our Abpromise guarantee covers the use of ab109094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 63.6 kDa). Preliminary experiments gave an approx. 23kDa band in Human Colon, Heart and Ovary lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kDa according to NP_001457.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificityWidely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • DomainLys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled homolog 2 antibody
    • Frizzled-2 antibody
    • Frizzled2 antibody
    • Fz 2 antibody
    • Fz-2 antibody
    • Fz2 antibody
    • FZD 2 antibody
    • FZD2 antibody
    • FZD2_HUMAN antibody
    • FzE 2 antibody
    • FzE2 antibody
    • hFz 2 antibody
    • hFz2 antibody
    • Mfz10 antibody
    see all

Anti-Frizzled 2 antibody images

  • ICC/IF image of ab109094 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells. The cells were then incubated with the antibody ab109094 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab109094 (2.5ug/ml) staining frizzled 2 in paraffin-embedded human thyroid tissue sections by Immunohistochemistry

References for Anti-Frizzled 2 antibody (ab109094)

ab109094 has not yet been referenced specifically in any publications.

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