• Product nameAnti-Frizzled 2 antibody
    See all Frizzled 2 primary antibodies
  • Description
    Rabbit polyclonal to Frizzled 2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the N-terminal region of human FZD2.

  • Positive control
    • Human PC-3, SKOV3, SW480 and HEK293 cell lysates for Western blotting and HepG2 for immunoprecipitation.



Our Abpromise guarantee covers the use of ab52565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 63 kDa). The observed molecular weight of ~ 85 kDa may be due to post-translational modifications such as glycosylation, which is predicted in the amino acid sequence.
IP Use at an assay dependent dilution. Use at a concentration of 7 µg/reaction.


  • FunctionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • Tissue specificityWidely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • DomainLys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled homolog 2 antibody
    • Frizzled-2 antibody
    • Frizzled2 antibody
    • Fz 2 antibody
    • Fz-2 antibody
    • Fz2 antibody
    • FZD 2 antibody
    • FZD2 antibody
    • FZD2_HUMAN antibody
    • FzE 2 antibody
    • FzE2 antibody
    • hFz 2 antibody
    • hFz2 antibody
    • Mfz10 antibody
    see all

References for Anti-Frizzled 2 antibody (ab52565)

This product has been referenced in:

See all 2 Publications for this product

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Thank you for your enquiry regarding ab52565.

This antibody has not been tested for functional or any blocking assay. As the storage buffer contains 0.1% sodium azide which is a cytotoxic agent thus ab52565 is unsuitable for cell culture &quo...

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Thank you for your inquiry and I sincerely apologize for the delay as I was awaiting more information from the supplying laboratory. The immunogen is a peptide derived from N-terminal region. We can say that the immunogen is in the first 75 amino ac...

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